猪乳exosome对仔猪肠道PEDV病毒的抑制作用及其机制研究

我国是养猪大国，仔猪肠道健康是生猪养殖的重要环节，猪流行性腹泻（PED）与仔猪肠道健康密切相关，对仔猪的病死率可达100%，该病目前尚无特效治疗药物，主要以综合防治为主，在预防过程中，母乳起着关键的作用。近年来，在母乳中发现存在大量的exosome，可在不同的细胞或组织间传递活性功能性物质，并调控靶组织或器官，但其作用机制和方式仍存在争议，然而迄今，仍未见乳exosome作用于PEDV的报道。本课题组前期发现，猪乳exosome对肠细胞感染PEDV具有抑制作用，但其作用机制及方式仍未揭示。因此，本项目拟通过体内和体外研究方法，运用形态学、分子生物学、细胞生物学和示踪检测等相关技术，深入探讨猪乳exosome抵抗仔猪肠道PEDV的调节机制，为揭示母乳成分增强仔猪对流行性腹泻抵抗力、提高仔猪成活率、改善仔猪肠道健康提供新的参考依据，同时为母子一体化研究和健康养殖提供新的思路和途径。

China is a large pig raising country. The health of intestine is the key factor for pig breeding. Porcine epizootic diarrhea is closely related to piglet intestinal health, which can lead to the fatality rate up to 100%. The disease has no specific treatment at present and relies on comprehensive prevention. Breast milk plays a capital role during the prevention process. For the past few years, many researchers reported that breast milk is abundant of exosomes, which can transfer functional molecules to different cells and tissues. However, their mechanism remains controversy. Up to now, there is still no report about the effects of milk exosomes on PEDV. Earlier, our group found that the porcine milk could inhibit the PEDV on intestinal cells, but the mechanism had not been revealed. Therefore, this program will investigate the regulatory mechanism of porcine milk on PEDV via the morphology, molecular biology and tracing methods in vitro and in vivo, which will provide new references of breast milk on enhancing the resistance of PEDV in piglets, improving survival rate and piglets’ intestinal health, and also provide new ideas and approaches for the research on mother-to-child integration and healthy breeding.