基于网络药理学及代谢组学技术的蒙药加味塔布森-2“肾主骨”辨证施治的药效物质基础及作用机制研究

蒙药复方整体药效并非单一成分药效的简单相加，研究方法有别于西药，应立足于整体观、动态观、辨证观。加味塔布森-2源于我国蒙古族骨科疾病的临床验方，方中各药多含补肾功效，符合“肾主骨”辨证施治理论，但作用机制尚不明确，制约其现代化、国际化发展。本项目拟采用网络药理学及代谢组学技术，结合中医辨证观，构建加味塔布森-2“肾主骨”辨证施治的药效物质基础及作用机制研究方法，开展研究如下：（1）通过网络药理学及代谢组学技术，解析加味塔布森-2“肾主骨”辨证施治“关键效应成分群”；（2）从关键效应成分群的“代谢处置”、对“内源性活性分子”调控、肾病与骨病疾病所共有的基因、生物进程及相关micro RNA几方面，探索加味塔布森-2“肾主骨”辨证施治作用特点及靶点；（3）揭示中医“肾主骨”理论与蒙医“骨枯病”治疗原则的内在物质及作用机制联系。本研究将为阐释符合蒙药复方多成分作用特点的研究模式提供新思路与方法。

The whole efficacy of Mongolian medicine is not a simple addition of single-component effects, and its research method is different from that of western medicine, which should be based on the overall, dynamic and dialectical view. The Modified Tabusen-2 was derived from the clinical prescription of the Mongolian orthopedic diseases, and there were various herbs had the invigorate kidney effects in this prescription, which followed syndrome treatment based on “kidney master bones theory”. However, the mechanism of this prescription was still unclear, that become the bottleneck of the prescription’s modernization and international. This project intends to use the network pharmacology and metabolomics technology, combined with the association between components, to construct a research method for pharmacodynamic substances and mechanism of mongolian medicine Modified Tabusen-2 applied in syndrome treatment based on “kidney master bones theory”. The research is as follows: (1) Analyze the “key effect component group” of Modified Tabusen-2 applied in syndrome treatment based on “kidney master bones theory” through network pharmacology and metabolomics technology; (2) Explore the characteristics and targets of Modified Tabusen-2 applied in syndrome treatment based on “kidney master bones theory” based on the study of “metabolism disposal” from key effect component groups, the regulation of “endogenous active molecules”, genes, biological processes and related microRNAs in both nephropathy and bone disease; (3) Discover the intrinsic substance and mechanism relationship between the “kidney master bones theory” in traditional Chinese medical science and the treatment principle of “Guku disease” in Mongolian medical science. This study will provide new ideas and methods for interpreting the Mongolian medicine mechanism that is consistent with the characteristics of interaction between multi-components.