3'UTR在mRNA稳定性、翻译效率及亚细胞定位等方面具有重要的作用。可选择性多聚腺苷酸化(APA)可产生具有不同长度3'UTR的mRNA异构体，与肿瘤、干细胞及胚胎发育、免疫等多种生物学现象具有密切关系。然而，APA影响这些生物学现象的分子机制尚不清楚。在该项目中，我们将通过细胞器分离、polysome profiling及高通量测序等技术，在全转录组水平上，系统地研究APA对于mRNA翻译效率及亚细胞定位的调控，鉴定APA调控mRNA翻译效率及亚细胞定位的规律，探讨其分子机制。在全转录组水平上研究APA的分子功能，将基因转录和翻译结合在一起，将有助于我们理解APA调控各种生物学现象的分子机制，为针对这些生物学现象开发新的干预手段提供基础，同时可加深我们对于分子生物学中心法则的认识。

3'UTR plays important roles in mRNA stability, translation efficiency and subcellular localization. Alternative polyadenylation (APA) can produce mRNA isoforms with different length of 3'UTRs and impact the molecular function of 3'UTR, and has been found related with several biological processes, such as tumor, stem cell and fetal development and immune responses. However, the molecular function of APA are still not clear. In this project, with isolation of organelles, high-throughput sequencing and polysome profiling, we will systematically investigate the regulation of APA on translation efficiency in transcriptome level to reveal the molecular funtion of APA, and we will also investigate its molecular mechanism. APA links gene transcription and translation, so this project will help us to understand the roles in biological processes and develop new intervening method for them, and it will also deepen the understanding of the molecular biology dogma.