近视是一种眼球过度发育性眼病。WnT是胚胎时期调控眼球发育的重要信号分子，在成年视网膜仍有表达并对眼球的生长起调节作用。课题组前期研究发现，小鼠形觉剥夺性近视（FDM）的视网膜中WnT2b、β-catenin及膜型卷曲蛋白5（MFRP5）表达上调，且与近视的程度呈正相关，但其在近视发生中的作用不清。为此，本项目分别采用基因芯片技术、免疫荧光、定量PCR及Western-blot等方法，检测FDM中WnT/MFRP各亚型的表达、表达部位及变化规律，观察其与近视发生的关系，并阐明其表达变化的机制；分别实施玻璃体腔注射WnT抑制剂DKK1和激活剂SB-2167631进行双向干预，观察其对近视的作用，分析WnT/β-catenin/MFRP、T细胞因子/淋巴细胞增强因子、视黄酸和转化生长因子β的表达，阐明调控WnT通路对近视的作用及其机制，为发现近视干预的可能有效靶点及研制新型抗近视药物奠定基础。

Myopia is an overdevelopment disease of the eye. WnT is an important signal molecule for the regulation of embryonic eye development. In mature retina, WnT still expresses and plays a role during the eye growth. Preliminary study of our research group founds that WnT2b/β-catenin and membrane frizzled protein(MFRP)5 is upregulated in the mice form deprivation myopia(FDM)and is positively correlated with myopia degree. However, the role of WnT/β-catenin/MFRP in the development of myopia still remains unclear. For this reason, our research project plan to use gene chip technology, immunofluorescence, RT-PCR and Western-blot method respectively to detect different isoforms of WnT/MFRP expression in FDM, as well as its expression sites and variation patterns, to observe its relationship with myopia development, to clarify its mechanism of the expression variation.Through DKK1( WnT blocker) and SB-2167631(WnT activator) intravitreal injection ,we compare the change of myopia with the control group, detect the expression of WnT/β-catenin/MFRP,T cell factor/lymphocyte enhancer factor, retinoic acid and transforming growth factor-beta, in order to clarify the effect and mechanism of myopia by regulation of the WnT pathway. We expect to provide a novel and effective target spot for myopia intervene and to lay the foundation for new anti-myopia drug. We hope it will possess scientific significance for the clinical prevention and treatment of myopia.