阻断或逆转胃癌癌前病变（PLGC）是预防胃癌发生的有效途径。中医药经验方“慢痞消”临床治疗PLGC效果显著，能从根本上逆转患者和PLGC模型大鼠胃黏膜损伤，但其药效物质基础和作用机制尚不清楚。PLGC的发生、发展及治疗与肠道菌群密切相关，“慢痞消”组方中药能调节肠道菌群平衡，改善机体免疫功能。“慢痞消”如何通过调节肠道菌群平衡来治疗PLGC，值得进一步探究。本项目在前期工作基础上，拟以中药与肠道菌群相互作用为切入点，结合血清药物化学、肠道菌群基因组学、代谢组学和体外细菌培养学方法，进一步探究“慢痞消”组方中药经肠道菌群代谢后的血中移行成分及其生物转化方式，探究其对PLGC模型大鼠肠道菌群结构组成、基因功能和代谢通路的影响，筛选组方中调节肠道菌群的中药有效部位和有效成分，以阐明其药效物质基础和作用机制，为其临床应用提供科学依据，为以调节肠道菌群平衡为靶标的中医药治疗PLGC研究提供新思路。

To block or reverse PLGC is an effective way to prevent gastric cancer. Manpixiao, a Chinese herbal formula based on clinical experience had significant clinical efficacy in the treatment of PLGC and significantly ameliorated the gastric mucosal injury in patients and PLGC model rats. However, it is still unclarified that the pharmacodynamic basis and mechanism of Manpixiao in the treatment of PLGC. It was reported that the occurrence ,development and treatment of PLGC was closely related to intestinal flora, and the Chinese herb medicines, the composition of Manpixiao, could regulate the intestinal flora balance and improve the systemic immune function. How does Manpixiao treat PLGC by regulating intestinal flora balance? which is worth further exploration. Therefore, based on our preliminary study, set the interaction between TCM and intestinal flora as the entry point, combined with the serum medicinal chemistry, intestinal flora genomics, metabolomics and in vitro microbial culture methods, we intend to further explore the components in the blood and their biotransformation methods of the Chinese herbal medicines in the Manpixiao, and the effect of Manpixiao on the structural composition, gene function and metabolic pathway of intestinal flora in PLGC model rats, and screen the effective portions and active ingredients of Manpixiao with regulating the intestinal flora, in order to clarify the pharmacodynamic basis and mechanism of Manpixiao in treatment of PLGC. It would provide the scientific basis for its clinical application, and new idea for the research on TCM in the treatment of PLGC with the target of regulating intestinal flora balance.