细菌MFS家族核苷转运蛋白的转运分子机制研究

核苷和核苷类似物作为重要的生物活性物质，被广泛的应用于一系列临床疾病治疗。核苷转运蛋白介导的的核苷跨膜转运对于核苷的发酵生产起到了重要调节作用。但截至目前，细菌MFS（major facilitator superfamily）家族核苷转运蛋白跨膜转运核苷的分子机制尚无报道。本研究拟通过对细菌MFS家族核苷转运蛋白的结构和生化性质进行深入研究，揭示核苷跨膜转运的分子机制，为通过修饰改造核苷转运系统调节核苷转运及提高发酵生产效率提供理论基础。

As a group of important bioactive molecules, nucleosides and nucleoside analogues, are widely used in therapeutic drugs, including a range of antiviral and anticancer products. Nucleoside transporters are the membrane proteins which mediate the transport of nucleosides across the plasma membrane, playing a key role in the microorganism fermentation process of producting nuleosides. However, up to date, the transport mechanism of bacterial MFS(major facilitator superfamily）nucleoside transporters is still elusive. To elucidate the molecular mechanism of nucleoside transport, we propose to systematically investigate the structural basis and biochemical features of bacterial MFS nucleoside transporters. Our research also aim to provide theoretical basis for regulating nucleoside transport by protein engineering, further improving the production efficiency of nucleosides.