肝细胞癌（HCC）在我国的发病率和死亡率高，尚缺乏安全有效的微创治疗方法。微波动力治疗（MWDT）是采用纳米材料介导低功率微波产生活性氧来治疗肿瘤的一种新方法。目前，可用于介导MWDT治疗HCC的纳米材料较少，亟需开发新的多功能纳米材料来实现对HCC病灶的靶向定位、成像引导和有效治疗。基于本课题组前期对多功能纳米材料制备及天然药物抗癌等研究，本项目拟设计合成一种Yolk-Shell结构型PHO@mTiO2/MnO2/Lien-GC33纳米颗粒。利用该纳米颗粒介导的靶向磁共振和超声造影成像分别于治疗前和治疗过程中实现对HCC病灶的精确定位及成像引导。同时，利用该纳米颗粒介导的MWDT治疗和莲心碱化疗联合低功率微波固有的热消融作用实现对HCC病灶的有效治疗，并从细胞周期和上皮间质转化的角度阐释其分子生物学机制，为该新型联合治疗方法在HCC中的应用提供理论依据和技术基础。

Hepatocellular carcinoma (HCC) has high morbidity and mortality in China. However, so far there lacks a safe and effective minimally invasive treatment for HCC. Microwave dynamic therapy (MWDT) is a new therapy that uses nanomaterials to mediate low power microwaves to produce reactive oxygen species for cancer treatment. At present, few nanomaterials can be employed to mediate the MWDT treatment in HCC, so it is urgent to develop novel multifunctional nanomaterials to achieve targeted localization, imaging guidance and effective treatment of HCC lesions. Based on our previous researches on the synthesis of multifunctional nanomaterials and the anticancer effects of natural drugs, this project intends to design and synthesize a kind of Yolk-Shell structured PHO@mTiO2/MnO2/Lien-GC33 nanoparticles. The nanoparticle-mediated targeted magnetic resonance imaging and contrast-enhanced ultrasound imaging will be used to achieve precise positioning and imaging guidance of HCC lesions before and during the treatment, respectively. Meanwhile, the nanoparticle-mediated MWDT and liensinine chemotherapy combined with the intrinsic thermal ablation function of low power microwave will be applied to achieve effective treatment for HCC. Additionally, molecular biological mechanisms related to the treatment will be elucidated from the perspective of cell cycle and epithelial-mesenchymal transition. These findings will provide theoretical basis and technological foundation for the application of the novel combined treatment in HCC.