生长分化因子-5修饰纳米结构磷酸钙磁性微载体制备及成骨活性研究

批准号:
82002289
项目类别:
青年科学基金项目
资助金额:
24.0 万元
负责人:
肖东琴
依托单位:
学科分类:
骨、关节、软组织损伤与修复
结题年份:
2023
批准年份:
2020
项目状态:
已结题
项目参与者:
肖东琴
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中文摘要
为从微纳米尺度上对模块组织构成与功能进行仿生,开发适用于骨组织修复所需的微载体材料,本研究提出设计制备具有磁性的多功能磷酸钙颗粒作为微载体,用于自下而上组织工程构建并利用磁性实现对模块单元的高效组装。前期研究中我们将Fe2+/Fe3+掺入磷酸钙晶体中,合成具有磁性的磷酸钙空心微球。本研究拟采用模板仿生技术制备不同纳米结构表面的磷酸钙磁性微球,通过微载体表面结构修饰促进细胞成骨分化;接着,将具有促血管化及新骨形成功能的生长因子GDF-5与双磷酸盐偶联,增强其与磷酸钙微载体的主动结合能力并实现生长因子在骨组织周围的有效释放。最后,将微载体与BMSCs复合,在外界磁场作用下组装形成块状组织,植入动物体内评价其异位成骨活性。本项目的实施有利于开发适用于硬组织修复并具有调控细胞响应功能的微载体材料,同时有助于从三维角度探索微载体表面结构及化学修饰促骨再生机制,为骨组织修复微环境构建提供参考。
英文摘要
In order to biomimic the structure and function of the modular tissue at the micro-nano scale and develop microcarrier materials suitable for bone tissue repair, this study proposes the design and preparation of magnetic multifunctional calcium phosphate particles as microcarriers for the fabrication of bottom-up tissue engineering and achieving efficient assembly of modular units by use of magnetism. In our previous studies, magnetic hollow calcium phosphate microspheres were synthesized by co-doping Fe2+ and Fe3+ into crystals. In this study, templated biomimetic method is used to prepare calcium phosphate microspheres with different nanostructured surfaces, for promoting cell osteogenic differentiation by modification of surface structure of microcarriers. Moreover, growth differentiation factor-5 (GDF-5) with enhanced vascularization and osteogenesis properties is coupled with bisphosphate, for enhancing its active binding ability with calcium phosphate magnetic microspheres and achieving effective release of growth factors around bone tissue. Finally, the prepared calcium phosphate magnetic microcarriers are combined with BMSCs, and further assembled into large tissue under the action of an external magnetic field, which are implanted into animals to evaluate their ectopic osteogenic activity. The implementation of this project is conducive to the development of microcarrier materials suitable for hard tissue repair and capable of regulation of cell response. Meanwhile, it is helpful to explore the osteogenic mechanism of surface structure and chemical modification of microcarrier from a three-dimensional perspective, which will provide references for building microenvironment for bone tissue repair.
期刊论文列表
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专利列表
DOI:10.1016/j.mtcomm.2022.104530
发表时间:2022-10
期刊:Materials Today Communications
影响因子:3.8
作者:Qiao Zhao;Dongqin Xiao;Yuwei Li;Xinghan Chen;Kun Hu;Xu-wei Luo;Fei Yang;Ze-long Yang;Kang Liu;G. Feng;Jinhui Liu;Daxiong Feng;K. Duan
通讯作者:Qiao Zhao;Dongqin Xiao;Yuwei Li;Xinghan Chen;Kun Hu;Xu-wei Luo;Fei Yang;Ze-long Yang;Kang Liu;G. Feng;Jinhui Liu;Daxiong Feng;K. Duan
DOI:--
发表时间:2022
期刊:中国组织工程研究
影响因子:--
作者:范好美;肖东琴;匙峰;罗栩伟;魏剑林;刘晋辉;赵菊花
通讯作者:赵菊花
DOI:--
发表时间:2023
期刊:中国组织工程研究
影响因子:--
作者:李立斯;张成栋;李小龙;叶姿妤;蒲超;杨在君;匙峰;肖东琴
通讯作者:肖东琴
DOI:10.1093/rb/rbab050
发表时间:2021-10
期刊:Regenerative biomaterials
影响因子:6.7
作者:Shi F;Xiao D;Zhang C;Zhi W;Liu Y;Weng J
通讯作者:Weng J
DOI:10.3390/jfb13030126
发表时间:2022-08-24
期刊:Journal of functional biomaterials
影响因子:4.8
作者:Luo X;Xiao D;Zhang C;Wang G
通讯作者:Wang G
国内基金
海外基金
