由大丽轮枝菌引起的作物黄萎病发生严重且难以防治，枯草芽胞杆菌NCD-2可有效减轻该病害的发生。前期发现，NCD-2菌株中群体感应相关突变体ΔcomX可显著降低对大丽轮枝菌的抑菌活性和微菌核的形成，同时NCD-2菌株发酵液中的ComX粗提物也具有抑制菌丝和微菌核的活性，推测NCD-2菌株中的信号分子ComX调控大丽轮枝菌的生长和微菌核形成。本研究拟进一步通过原核表达获得ComX信号分子，明确ComX对大丽轮枝菌生长和微菌核形成的调控作用；联合利用转录组和蛋白组获得大丽轮枝菌中受ComX诱导并且与菌丝生长及微菌核形成相关基因；通过对目的基因进行功能分析，验证其在大丽轮枝菌生长及微菌核形成的作用，并通过等温滴定量热法探究ComX与目的蛋白的互作关系。研究结果在理论上将丰富芽胞杆菌群体感应信号分子ComX在跨界调控真菌代谢方面的新功能，在实践上抑制微菌核的产生、降低病原菌数量从而达到控制病害的目的，为植物真菌病害的防治提供新思路。

Verticillium wilt of crop caused by Verticillium dahliae is serious and difficult to control. Bacillus subtilis NCD-2 could effectively reduce the occurrence of this disease. Previous study showed that the comX mutant of strain B. subtilis NCD-2 not only reduced the antifungal activity against Verticillium dahliae, but also decreased the microsclerotia formation of V. dahliae. The ComX extracted from NCD-2 strain supernatant also showed the same effect. Therefore, it`s speculated that ComX secreted by NCD-2 strain regulated the growth and metabolism of V. dahliae. To further explore the mechanism of ComX regulating the growth and microsclerotia formation of V. dahlia, the purified ComX would be obtained by heterologous expression, and the effect of ComX on the antifungal activity and microsclerotia formation would be tested. Then, the genes involved in V. dahlia growth and microsclerotia formation would be selected by transcriptome and proteome, and the function of selected genes would be confirmed by molecular genetic methods. Finally, the interaction between the ComX and the protein encoded by targeted genes would be clarified by isothermal titration calorimetry. This project would theoretically enrich the new function of the quorum sensing signal molecule ComX in interkingdom communication, inhibiting the production of microsclerotia and reducing the number of pathogens in practice to achieve the purpose of controlling diseases，as well as provide a new insight for the biocontrol of phytopathogens.