开花是植物从营养生长向生殖生长转变的一个重要事件。拟南芥SVP与AGL24 （AGAMOUS-LIKE24）序列非常相似，但在调控成花方面功能相反。系统发育分析表明在MADS-box中选择压力作用位点的改变是导致SVP与AGL24分化的原因之一。本研究在山核桃中已克隆了两个选择压力作用位点存在差异的AGL24基因CcAGL24a和CcAGL24b。本项目拟通过过表达拟南芥野生型和agl24突变体分别验证CcAGL24a与b的功能。利用qPCR、原位杂交和WB分析CcAGL24a/b在山核桃中的时空表达与成花的关系；通过亚细胞定位和转录激活实验分析CcAGL24a/b中的核定位和发挥激活作用的结构域；采用Y2H筛选CcAGL24a/b的互作蛋白，以及挖掘其下游调控基因。通过本研究期望在转录和蛋白水平上解析CcAGL24a和CcAGL24b调控成花的分子机理，为山核桃成花调控机制提供新的观点。

Flowering is an important event in the transition from vegetative to reproductive growth in plants. The SVP sequence is very similar to AGL24 (AGAMOUS-LIKE24) while the function in controlling Arabidopsis flowering is opposite.Phylogenetic analysis showed that the changes of these selection pressures sites in MADS-box are one of the important reasons for the differentiation of SVP gene with AGL24. In this study, two AGL24 genes, CcAGL24a and CcAGL24b, were successfully cloned from hickory and their selective pressure sites varied. Here we will verify CcAGL24a/b function through over-expression in wild type and agl24 mutants respectively. Then qPCR, in situ hybridization analysis and Western Blot will be used to reveal the relationship between the temporal and spatial expression of CcAGL24a/b and flowering, and subcellular localization and transcriptional activation assay, respectively, to analyze the domains of nuclear localization and activation activity in CcAGL24a/b. Furthermore, the interacting proteins of CcAGL24a/b will be screened by yeast two-hybrid system, and the target genes of CcAGL24a/b will be mined. The implementation of this project will reveal the molecular mechanism of CcAGL24a and CcAGL24b involved in the regulation of flowering at the transcription and protein levels, and provide a new insight for the regulation mechanism in hickory flowering.