响应干旱胁迫的马铃薯MAPK基因筛选鉴定及信号转导通路解析

促分裂原活化蛋白激酶（MAPK）级联途径是真核生物中普遍存在的、高度保守的信号转导模式。在马铃薯中，StMAPKs家族的基因功能及信号转导机制目前尚不清楚。在前期研究中，我们通过生物信息学鉴定了15个马铃薯StMAPKs家族基因，并筛选了与抗旱相关的3个StMAPKs基因。本研究将对马铃薯进行干旱胁迫，进一步筛选鉴定干旱胁迫相关的马铃薯StMAPKs基因；并对筛选出的干旱胁迫相关StMAPKs基因进行克隆、亚细胞定位和基因功能鉴定；用酵母双杂交与双分子荧光互补技术，挖掘马铃薯响应干旱胁迫的StMAPKs上游的StMAPKKs蛋白和下游的互作转录因子及功能蛋白，阐明马铃薯MAPK级联途径对干旱胁迫信号分子的响应机制和代谢通路，为利用信号转导途径调控马铃薯的抗旱性提供理论依据。

Mitogen-activated protein kinases (MAPK) cascades are a highly conservative signal transduction model, which are commonly found in eukaryotes. However, StMAPK gene functions and signal transduction pathways are still unknown in potato. In our previous studies, we identified 15 MAPKs family genes by bioinformatics and screened out three drought-related StMAPK genes in potato under drought stress. Based on the study, the potato plants will be under drought stress to screen out and identify drought-related MAPK genes. In addition, we will screen the upstream StMAPKKs and downstream interacting protein which are related to StMAPKs by the yeast two-hybrid system and bimolecular fluorescence complementation. The aim of this research is to elucidate the signal molecules of the MAPK cascade pathway of potato under drought stress, and provide theoretical foundation for comprehensive resistance in improving drought resistance of potato by cell signal transduction regulation.