动脉粥样硬化斑块纤维化程度的提高，促进了血管痉挛及急性心脑血管事件的发生率; 心肌梗死后心肌纤维化的形成，严重阻碍了心肌梗死患者的心肌再灌注治疗。精准靶向评估动脉粥样硬化斑块及心肌梗死组织中的纤维化程度，具有重要的临床意义。本项目组前期研究成功证实了MR靶向合成型血管平滑肌细胞及Tenascin-C蛋白表达的可行性。研究证实，TN-X是动脉粥样硬化斑块与急性心肌缺血梗死组织纤维化重构的重要调节蛋白。是理想的分子影像学靶点。本项目拟以TN-X为研究靶点，制备特异性靶向TN-X的抗体探针；建立ApoE-小鼠动脉粥样硬化斑块模型及大鼠急性心肌缺血梗死性纤维化模型。MR成像检测粥样硬化斑块及心肌缺血梗死组织中TN-X的表达，与病理学及分子生物学研究结果对比分析。探索MR靶向成像在动脉粥样硬化斑块及急性心肌梗死组织纤维化重构精准评估的可行性，促进动脉粥样硬化性心血管疾病的防治水平。

Although fibrosis is becoming increasingly recognized as a major cause of morbidity and mortality in atherosclerotic diseases and cardiac myocardial infarction disease, available treatment strategies are limited. Targeted imaging for the fibrosis degree in atherosclerosis plaque and cardiac infarction are needed. This research group has been year’s study of MR molecular imaging in atherosclerosis, and have been succeeded in MR targeted imaging for the proliferated smooth muscle cells (SMCs) and Tenascin-C. Tenascin X proteins is an important factor in fibrosis development and make them attractive both as biological markers and as targets for therapeutic intervention for atherosclerosis and myocardial infarction fibrosis reconstruction. Therefore, based on the previous research work, this research project is designed to develop the typical Fe2O3/DMSA-TN-X antibody MR imaging probe, and to perform the in vitro and in vivo MR imaging study for the express of TN-X in atherosclerosis ApoE-mouse model and acute myocardial infarction fibrosis rats’ model separately. We wish for make the basic research to identify the fibrosis formation process of the atherosclerotic plaque and the infarction of myocardium in vivo with MR imaging, and to improve the diagnosis and clinical treatment methods for the cardiovascular disease.