诱导排卵因子OIF/βNGF介导PAK1促进双峰驼LH生成和诱导排卵的分子机理

目前双峰驼独特的繁殖机理—精清诱导排卵机制尚不完全清楚。我们前期的研究结果表明精清OIF/βNGF可诱导母驼产生LH排卵峰并促进其排卵，且诱导后母驼性腺轴组织和血液中PAK1表达显著上调，据此推测精清OIF/βNGF介导PAK1促进LH生成和诱导排卵，但其具体作用机制尚不清楚。本研究拟建立精清OIF/βNGF诱导母驼排卵模型，阐明PAK1表达与LH生成和诱导排卵的相关性；随后建立过表达/敲低PAK1细胞模型，联合精清OIF/βNGF诱导，解析PAK1促进LH生成的分子机理；最后家兔卵巢活体转染过表达和敲低PAK1载体，建立家兔诱导排卵模型验证OIF/βNGF介导PAK1促进LH生成和诱导排卵的作用机制。本研究将有助于揭示双峰驼诱导排卵的分子机理，为双峰驼及其他诱导排卵型动物繁殖机理的研究提供重要依据。

The unique reproductive mechanism of ovulation induced by seminal plasma in bactrian camel is not properly appreciated. Our previous study showed that OIF/βNGF in seminal plasma could induce the female camel to produce LH peak and promote its ovulation. And the expression level of PAK1 in the gonadal axis and blood of the female camel was significantly up-regulated after induction by OIF/βNGF. We hypothesized that OIF/ NGF promotes LH generation and ovulation induction via mediated PAK1, however the specific mechanism of this process is still unknown. In the present study, the ovulation model induced by OIF/βNGF will be constructed for elucidating the correlation between PAK1 expression and LH generation and ovulation induction. Subsequently, different cell models of PAK1 over-expression or knock down combined with OIF/βNGF inducing will be constructed for illuminating the molecular mechanism of PAK1 promoting LH generation. Finally, transfected these vectors to rabbit ovaries in vivo and constructed the rabbit ovulation induction model for validating the mechanism of PAK1 promoting LH generation and ovulation induction. This study will help to reveal the molecular mechanism of ovulation induction in bactrian camel and other ovulation-inducing animals.