双峰驼精清诱导排卵的分子调控机理

近年来双峰驼数量急剧下降，其主要原因是双峰驼繁殖性能低、周期长和速度慢，特别是双峰驼排卵的分子机理完全不清楚。课题组早期发现并报道双峰驼为精液诱导排卵动物，母驼肌肉注射精清24h后即开始排卵，36-48h完成排卵。在此基础上，本项目通过全基因组重测序和蛋白质组学的方法筛选诱导排卵过程中关键的靶基因和蛋白，并验证其表达规律，构建其基因-蛋白互调控作网络，进一步解析双峰驼精清诱导排卵的分子机理。为今后双峰驼和诱导性排卵动物繁殖及生殖相关疾病的研究提供依据。

The population of bactrian camel has a dramatic decline in recent years, the main reasons caused this situation is the lower reproductive performance, longer growth period and a slower growth rate, especially the completely unclear molecular regulation mechanism of bactrian camel seminal plasma induced ovulation. Early studies of our tern find and reported that the bactrian camel is a kind of semen induced ovulation animal. The female bactrian camel will begin to ovulation after intramuscular injecting seminal plasma for 24 hours, complete ovulation for 36-48 hours. Whole genome sequencing and proteomics approach were implemented in this project for screening key target genes and proteins in induced ovulation, verified the expression pattern and constructed the network of candidate genes and proteins based on our previous study. And further clarified the molecular regulation mechanism of bactrian camel seminal plasma induced ovulation. Which will provid the theoretical basis for bactrian camel and other induced ovulation animals in breeding and reproduction related diseases.