微生物降解是环境中多环芳烃生物修复的主要途径。微生物中，sRNA作为一种可在各种代谢途径及生理活动中起重要作用的转录后调控因子，近年来成为国际上的研究热点，但对sRNA在多环芳烃降解中的调控功能还未见报道。红球菌P14是一种可高效降解多种多环芳烃的海洋细菌，前期课题组对其基因组及多环芳烃降解相关基因进行了深入研究。本项目以红球菌P14为研究对象，利用dRNA-seq和Northern Blot等技术，预测并鉴定与一种多环芳烃化合物芘降解相关的sRNA，进一步通过qPCR与EMSA等技术，明确sRNA对芘降解相关靶基因的调控作用，从而阐明sRNA在红球菌P14多环芳烃降解中的调控功能。预期成果可为阐明多环芳烃降解机制提供理论基础。

Microbial degradation is usually considered as the main pathway of bioremediation of the polycyclicaromatic hydrocarbons (PAHs) in the environment. sRNA, as a post-transcriptional regulator which plays an important role in various metabolic pathways and physiological activities in microorganisms, has become a hot scientific topic in the current world, however, the function of sRNAs in the degradation of PAHs is still unknown. Rhodococcus sp. P14 is a bacterium from ocean with a strong ability of degradation of many PAHs. The genome and genes with functions in PAHs degradation were studied previously. In this project, Rhodococcus sp 14 is used as the object. This project intends to predict and identify sRNAs with the potential functions in the degradation of pyrene which is a kind of PAHs compounds through the use of dRNA-seq as well as Northern Blot. Moreover, qPCR and EMSA are used to clarify the functions of sRNAs on their target genes which are related to the pyrene degradation. Finally, this project intends to illustrate the role of sRNAs in Rhodococcus sp. P14 during PAHs degradation. The results will help to reveal the molecular mechanism of the degradation of PAHs.