MiR-206通过脂类代谢影响HCV感染和致病的机制研究

丙型肝炎病毒(HCV)感染是导致丙型肝炎的致病因素，由于其感染和致病过程复杂，目前尚未完全阐明具体的致病机制。已有研究表明HCV的感染、复制与致病过程均需要脂代谢蛋白的参与，与脂类代谢过程有着密切的关系；而microRNA 206 (miR-206) 近期被证明参与到肝组织脂类代谢过程中并发挥重要作用，因此推断miR-206可能通过脂类代谢途径影响HCV在宿主体内的感染、复制和致病的过程。本项目通过预测并确定miR-206调控的脂代谢相关基因，检测miR-206及其调控基因在HCV感染细胞中的表达改变情况；同时分析miR-206高表达或抑制后，对HCV感染和致病能力的影响，并确定其作用的脂代谢通路。通过综合分析，最终确定miR-206如何通过脂代谢途径影响HCV的感染和致病过程，构建功能网络图，进一步阐明HCV的感染和致病机制，并为HCV感染者临床治疗生物新药的研发奠定理论基础。

Hepatitis C virus (HCV) infection was the pathogenic cause of hepatitis C. Because of the complexity of its infected process and pathogenesis, the pathogenic mechanisms were not completely clear. It was reported that lipid metabolism participated in HCV infection, duplication, and pathogenesis, so HCV infection closely related with lipid metabolism. microRNA 206 (miR-206) was identified to be one important factor in lipid metabolism in liver, so we conferred that miR-206 might be affected HCV infection, duplication, and pathogenic process by lipid metabolism in liver. Firstly, we forecast target genes of miR-206 and validate them; secondly, mRNA and protein of miR-206 and its target genes were detected in cells with HCV infection. At same time, the ability of HCV infection and pathogenic process was detected after overexpressing and silencing miR-206. After comprehensive analysis, we construct the functional network of these genes to further clarify the role of miR-206 in HCV infected and pathogenic process by lipid metabolism. Our study will further improve the pathogenic mechanisms of HCV infection, and provide theoretical basis for newly biological drug for HCV treatment in clinic.