基于“煨脓长肉”理论从Notch通路调节巨噬细胞角度探讨紫朱软膏影响糖尿病溃疡愈合的机制

创伤过程中炎症反应状态异常可导致溃疡难愈，巨噬细胞功能失调对溃疡的影响尤为突出。Notch信号参与调控巨噬细胞介导的炎症反应。前期研究提示紫朱软膏可改善糖尿病溃疡早期炎症反应，促进创面愈合；基因测序提示愈合机制中伴随Notch通路的高表达。中医外治法理论中认为“煨脓长肉”可促进溃疡愈合，那么煨脓长肉中药紫朱软膏是否可以通过调控Notch通路调节巨噬细胞表达？其分子机制是什么呢？本研究拟通过体内外实验，借助Notch信号通路激动剂、抑制剂及Notch基因敲除的动物模型，观察紫朱软膏对Notch信号通路关键因子、巨噬细胞活性功能表达的影响，并初步探讨其机制，为煨脓长肉理论治疗糖尿病溃疡提供新的实验依据。

The abnormal state of inflammation can lead to ulcers non-healing in trauma, macrophage dysfunction effects on ulcer is particularly prominent. Notch signaling is involved in the regulation of macrophage mediated inflammatory response. Previous studies have suggested that the ointment can improve the early inflammatory reaction and promote wound healing of diabetic ulcers, and gene sequencing suggests that the Notch pathway is highly expressed in the healing mechanism. The theory of external therapy of traditional Chinese medicine that "simmer pus can promote the healing of ulcer, then simmer pus medicine Zizhu ointment can regulate macrophage expression by regulating Notch pathway? What is the molecular mechanism? In this study the in vitro and in vivo experiments with Notch pathway inhibitors and Notch agonists, gene knockout animal model in addition, observe the effect of Zizhu ointment on the expression of key factors, Notch signaling pathway activity of macrophage function, and to explore its mechanism, to provide new experimental evidence for simmer pus theory in the treatment of diabetic ulcers.