昆虫烟碱型乙酰胆碱受体（nAChR）是重要的杀虫剂靶标，其与杀虫剂互作的研究对于新农药创制、害虫防治和抗性治理等具有重要意义。研究发现Ly-6/neurotoxin（Lynx）能通过调控杀虫剂与nAChR的互作提高杀虫剂的药效，但机制尚不明确。因此，本项目在已发现东亚飞蝗多达11个Lynx的基础上推进以下研究：通过外源表达和电生理实验明确Lynx的调控功能差异与选择性；结合分子模拟及同源模建，明确Lynx空间结构与功能的关联；建立Lynx与nAChR互作的分子模型并通过氨基酸定点突变等验证Lynx与nAChR互作的关键氨基酸位点。本项目有望阐明Lynx对杀虫剂与nAChR互作的调控机制，深化对nAChR互作蛋白的功能认识，为实现全昆虫亚基nAChR的体外功能表达提供理论基础和实验支撑。同时，明确Lynx对杀虫剂与nAChR互作的调控机制，有利于以Lynx及其调控过程为潜在靶标的杀虫剂创制。

Insect nicotinic acetylcholine receptors (nAChRs) are important targets for many kinds of insecticides, including neonicotinoids, spinosyns and nereistoxin analogues. It is hoped that studies on the mechanisms of interaction of insect nAChRs and insecticides will help in the development of novel insecticides and in their rational use and resistance management. Previous studies found that Ly-6/neurotoxin (Lynx) could improve the efficacy of insecticides by regulating the interaction of insecticides and nAChRs, nevertheless the mechanism was unclear. Thus, in present study, based on as many as 11 potential Lynx have been found and cloned, the following research will be performed 1) clarifying the regulating capability and selectivity of lynx by using heterogenous expression system and electrophysiological system, 2) uncovering the relativity of spatial structure and function of lynx with molecular simulation and protein homology modeling, 3) establishing molecular model of interaction of Lynx and nAChR by molecular docking and validating the key amino acid of lynx for the interaction by site-directed mutagenesis. The project is expected to reveal the mechanism of Lynx regulating interaction of insecticides and nAChR, to get an insight into the function of associated proteins of nAChRs and to provide theoretical basis and practical experience for the functional expression of nAChRs with only insect subunits. Meanwhile, it is conducive to develop insecticides potentially targeting the lynx or the regulative process of lynx.