日灼是制约了枇杷产业的健康发展的重要障碍，枇杷抗日灼相关基因的研究和利用对于稳定枇杷产量，提高果实品质具有重要意义。本项目拟在获得了响应日灼的EjWRKY基因的基础上，利用荧光定量PCR技术分析EjWRKY基因在枇杷不同组织和不同发育阶段果实的表达模式；利用亚细胞定位法确定WRKY转录因子行使功能的细胞器；同时，构建 RNAi敲除和过表达EjWRKY基因表达载体，转化番茄进行功能分析；应用酵母单杂交筛选鉴定EjWRKY转录因子的下游功能基因，并利用荧光定量PCR分析EjWRKY及其调控的功能基因在日灼条件下的互作模式。本项目不仅对阐明日灼条件下EjWRKY基因在枇杷果实中的转录调控的机制具有重要意义，也为提高枇杷乃至其他果树果实日灼耐受性和培育抗日灼的果树新品种奠定了一定的理论基础。

Sunburn is an environmental factor that greatly restricts loquat industry sustainable development. The research and utilization of sunburn tolerance related genes in loquat is important to improve fruit yield and quality. Based on the obtained sunburn-response EjWRKY genes, this project plans to analyze the expression pattern in different organizations and fruit development periods of loquat by using RT-PCR, and subcellular location method is used to confirm the function organelles of WRKY. Then construct the RNAi and over-expression vectors and make genetic transformation in tomato with gene functional analysis. The downstream functional genes of EjWRKY will be screened using the yeast one-hybrid system and the interaction model will be analyzed by RT-PCR in sunburn stress. This project could not only be great significance to clarify the transcriptional regulation mechanism of EjWRKY gene under sunburn condition, but also provide the theoretical basis on improving fruit tolerance and breeding resistant varieties in loquat even other fruit trees.