子痫前期（preeclampsia，PE）是一种发生在妊娠中晚期的妊娠期特有的疾病，是导致孕产妇和围产儿发病及死亡的主要原因之一。母胎界面免疫功能和胎盘功能的异常都是导致子痫前期的出现的重要因素，但其确切的发病机制仍有待研究阐明。NRSF是一种去组蛋白乙酰化修饰蛋白，对靶基因的转录有负调控作用，但其在生殖调控中的作用目前尚无明确报道。已发现VEGF的膜受体NRP1是NRSF的一个靶基因，而VEGF是调控血管收缩舒张以及增殖的一个重要因子，由此推测，NRSF/NRP1/VEGF调控途径可能在PE发生发展过程中发挥重要作用。本项目拟通过对人和小鼠胎盘组织滋养层细胞中NRSF、NRP1和VEGF的表达模式，以及NRSF表达水平的变化对滋养层细胞活性的影响等进行研究分析，探讨NRSF在PE发生发展中的作用及其分子机制，以期为PE临床防治技术的研究提供科学依据。

Preeclampsia (PE) is a disease specific occurs in middle or late pregnancy, which could result in maternal and perinatal morbidity and mortality. The immune system abnormalities in maternal-fetal interface and placental function confusion are both important factors leading to the preeclampsia, which mechanism remains to be elucidated. NRSF is a histone acetylation modification protein, which could negative control some target genes, but the role of NRSF in the reproduction has not been reported yet. It is well known that the VEGF’s membrane receptor NRP1 was the target of NRSF. And it has been reported that VEGF is one of the important factor which could regulate systolic and diastolic blood vessel proliferation.Thus, we assume that the pathway of NRSF/NRP1/VEGF might play a role in the development of PE. This project intends to study the expression pattern of NRSF, NRP1/VEGF in the human placental trophoblastic cells, and the affect of NRSF variation in trophoblast cells. We also intend to study the role of NRSF and its molecular mechanism in PE, which could provide a scientific basis for prevention and treatment of PE technology research.