寄生蜂PDVs对寄主小菜蛾脑神经肽的调控

以携带不同类型多DNA病毒（PDVs）的2种内寄生蜂（菜蛾盘绒茧蜂和半闭弯尾姬蜂）寄生同一寄主（小菜蛾）为研究体系，在分析和鉴定寄主小菜蛾与变态发育相关的主要脑神经肽（促前胸腺激素PTTH、咽侧体活化激素allatotropin、咽侧体抑制肽allatostatin、糅化激素bursicon等）的基础上，研究菜蛾盘绒茧蜂病毒（CvBV）和半闭弯尾姬蜂病毒（DsIV）对寄主主要脑神经肽表达的影响，鉴定CvBV和DsIV中影响主要脑神经肽基因表达的关键基因及他们的产物，分析其功能及其影响的信号途径，从而提出不同类型PDVs(CvBV和DsIV)调控寄主小菜蛾生长发育的途径和模式，解析不同类型PDVs作用的分子机制，开辟寄生蜂PDVs调控寄主的分子机理研究的新领域，为最终阐明PDVs与其它寄生因子协同发挥作用调控寄主生理的机制及发展持续控制害虫的新技术奠定扎实的基础。

We use the diamondback moth, Plutella xylostella, and its two solitary parasitoids, Cotesia vestalis (Braconidae) and Diadegma semiclausum (Ichneumonidae), as a model system to examine the regulation patterns of major neuropeptides, such as PTTH, allatotropin, allatostatin and bursicon, of host larvae by two types of polydnaviruses, Cotesia vestalis bracovirus (CvBV) and Diadegma semiclausum ichnovirus (DsIV) and the molecular mechanism underlined. Firstly we will characterize the genes and gene transcripts of these neuropeptides at different developmental stages of host larvae and their localization in brain cells. Secondly we will examine the impact on the expression of these neuropeptides by CvBV and DsIV. Lastly, we will identify the key genes and their products of CvBV and DsIV that are interacted with these neuropeptides in host brain and the possible regulating pathways where they are involved. The results will not only reveal the regulation mechanism of different types of polydnaviruses on host physiology, but also provide us a profound base for understanding mechanism underlying the co-regulation of polydnaviruses and other parasitiod-associated factors on host physiology, and for developing new techniques for sustainable management of insect pests.