伏马毒素作为主要的真菌毒素之一，不仅能导致许多人畜疾病，还与真菌侵染相关。因此，探索伏马毒素生物合成的分子调控机制对减少真菌病害和真菌毒素污染，确保食品安全具有很重要的理论和实际意义。LaeA转录因子在丝状真菌次生代谢物生物合成和侵染过程中发挥重要的调控作用。申请人前期研究发现，不同碳源处理能够影响层出镰刀菌伏马毒素产量和LaeA的表达。本项目以香蕉采后重要致病菌层出镰刀菌为材料，研究LaeA对伏马毒素生物合成的转录调控作用，主要内容包括：1）鉴定对伏马毒素生物合成起转录调控的LaeA；2）分析层出镰刀菌LaeA的转录调控特性和调控网络；3）LaeA的功能验证；4）确定伏马毒素与层出镰刀菌侵染的关系。研究结果有望从转录调控角度阐明伏马毒素生物合成的分子调控机制，并初步明确伏马毒素的生物学功能，完善伏马毒素生物合成的调控机制和层出镰刀菌的侵染机制，为提出控制毒素残留和真菌侵染提供理论依据。

Fumonisins, as one of the major mycotoxins, are not only considered to be a carcinogen and tumor activator in animal and human, but also involved with fungal infection. Therefore, clarifying its biological formation and regulation mechanism has theoretical and practical significance in reducing fungal disease and mycotoxin contamination risk and ensuring food safety. LaeA transcription factors (TFs) have been proven to play an essential role in the regulation of secondary metabolism and virulence of filamentous fungi. Our previous research have found that different carbon sources could regulate fumonisin biosynthesis and expressions of some LaeA genes. The project is to investigate transcriptional regulation of FUM by LaeA during fumonisin biosynthesis using Fusarium proliferatum, the most serious pathogenic fungi of banana fruit. The following issues will be studied: 1) identification of LaeA which possibly regulate the transcription of fumonisin biosynthesis related genes; 2) analysis of the regulation properties and regulation network of the identified LaeA; 3) verification of the role of LaeA using gene knock-out technology; 4) confirming the relationship between fumonisin production and infection ability of Fusarium proliferatum. The results will contribute to illuminate the molecular mechanism of fumonisin biosynthesis from the viewpoint of transcriptional regulation and provide some basic information for the biological role of fumonisin. Meanwhile, the results will improve our understanding of the regulation mechanism of fumonisin biosynthesis and infection mechanism of Fusarium proliferatum. In all, the results are useful in providing theoretical basis for the proposed new control strategies of mycotoxin contamination and fungal infection.