白芍具有养血柔肝、健脾益气功效，临床上常用于治疗免疫系统疾病。申请人前期研究发现，白芍具有抗过敏性哮喘作用，且麸炒后疗效增强，但其作用的物质基础和机理尚未明确。申请人研究发现，白芍麸炒后化学组分发生变化，且能够降低P-Gab2，P-Akt和P-Erk蛋白表达水平，抑制肥大细胞脱颗粒和钙流释放。因此提出科学假说“白芍麸炒后体内外化学组分发了转化，进而调控Gab2/AKT/Erk信号通路关键因子影响囊泡转运，抑制钙流和肥大细胞脱颗粒，发挥抑制过敏性哮喘增效作用”。本项目运用基因修饰、谱效关系和代谢组学等技术，通过整体辨识和靶标分析，结合体内物质基础，揭示白芍麸炒前后体内成分与代谢经路靶点的相关性，并探究其对Gab2/AKT/Erk信号关键因子调控作用，阐明白芍麸炒增强抗过敏性哮喘的物质基础和多靶点协同作用机制。本研究不仅有助于阐明白芍麸炒增效机制，同时也为白芍抗过敏性哮喘药物开发提供参考依据。

Bran fryed Radix Paeoniae Alba have helps calming the liver and spleen tonic effect. It has significant curative effects to applying the Chinese medicine theory of Radix Paeoniae Alba to cure immune system diseases. Previous study discovered enhance the inhibitory effect on allergic asthma rat after processed. and until now there is no research on the mechanism of Chinese herbal pieces effect changes. In our preliminary experiment, Radix Paeoniae Alba after bran fried decrease of the expression of P-Gab2, P-Akt and P-Erk, and inhibit the mast cell degranulation and calcium release. Based on the previous researches, this subject advance hypothesis that “Radix Paeoniae Alba after bran fried through the regulation of Gab2/AKT/Erk signaling pathway to inhibit vesicles transport, mast cell degranulation and calcium release, thereby enhancing its anti-allergic asthma synergistic effect”. This subject through discuss the regulatory effect of overall metabolite and the regulation of key targets, using gene knockout, spectrum-effect relationship, metabonomics and other technology innovatively to study the correlation between the main constituents in vivo and the multitargeted of metabolic. By exploring the regulation of Gab2/AKT/Erk signaling pathway to clear the anti-allergic asthma active substance and reveal the multi-target synergistic mechanism of bran fried Radix Paeoniae Alba. This project provides the basis for the clinical application of bran fried Radix Paeoniae Alba and new thought for the traditional Chinese medicine processing theory research.