Screening of Bioactive Fraction of Radix Paeoniae Alba and Enhancing Anti-Allergic Asthma by Stir-Frying Through Regulating PI3K/AKT Signaling Pathway.
Screening of Bioactive Fraction of Radix Paeoniae Alba and Enhancing Anti-Allergic Asthma by Stir-Frying Through Regulating PI3K/AKT Signaling Pathway.
复制标题
白芍生物活性部位的筛选及炒用调节PI3K/AKT信号通路增强抗过敏平喘作用
DOI：
10.3389/fphar.2022.863403
复制
发表时间：
2022
影响因子：
5.6
通讯作者：
Cao, Gang
中科院分区：
文献类型：
--
作者：
Sang, Xia'nan;Ying, Jialiang;Wan, Xuedong;Han, Xin;Shan, Qiyuan;Lyu, Qiang;Yang, Qiao;Wang, Kuilong;Hao, Min;Liu, Erlong;Cao, Gang
过敏性哮喘是一种常见的呼吸道炎症性疾病。白芍粗品（RPA）及其加工品在传统医学中经常被用作解热和抗炎剂。为了评价蜜麸加工的效果，在卵清蛋白（OVA）诱导的小鼠模型中使用不同部位的RPA治疗抗过敏性哮喘，然后将RPA最有效部位与蜜麸炒白芍（FRPA）治疗抗过敏性哮喘的药理作用进行相互比较。结果表明，RPA二氯甲烷馏分处理可显着改善肺组织病理状况，支气管肺泡灌洗液（BALF）中嗜酸性粒细胞等细胞数量减少，多种炎症因子表达增加。此外，研究发现，与高剂量二氯甲烷RPA组分相比，高剂量二氯甲烷FRPA组分治疗可以改善肺部病理状况。此外，FRPA 还可减少炎症因子的表达和 PI3K/AKT 信号通路的磷酸化。最后，通过UPLC/Q-TOF-MS分析，FRPA二氯甲烷馏分中含量差异显着的化合物为芍药苷、没食子酸乙酯、五没食子酰葡萄糖、没食子酰芍药苷等。这些结果表明FRPA的二氯甲烷部分具有抗过敏性哮喘的增强作用，为探索RPA的加工机制提供了实验基础。
Allergic asthma is a common respiratory inflammation disease. The crude Radix Paeoniae Alba (RPA) and its processed products have been used frequently as antipyretic and anti-inflammatory agents in traditional medicine. To evaluate the effect of honey and bran processing, different fractions of RPA were used for treating anti-allergic asthma in the ovalbumin (OVA)-induced mice model, and then, the most effective fraction of RPA and stir-frying Radix Paeoniae Alba with honey and bran (FRPA) for treating anti-allergic asthma were compared mutually for pharmacological effects. The results showed that the treatment of the dichloromethane fraction of RPA significantly improved the pathological condition of lung tissues, decreased the number of eosinophils and other cells in bronchoalveolar lavage fluid (BALF), and the increased the expression of various inflammatory factors. Furthermore, the study discovered that the lung pathological conditions, compared with the high dose of dichloromethane RPA fraction, could be ameliorated by high dose of dichloromethane FRPA fraction treatment. Moreover, the expression of inflammatory factors and the phosphorylation of the PI3K/AKT signaling pathway could be diminished by FRPA. Finally, the contents of compounds with a significant difference in the FRPA dichloromethane fraction were paeoniflorin, ethyl gallate, pentagalloylglucose, galloylpaeoniflorin, and others by UPLC/Q-TOF-MS analysis. These findings suggest that the dichloromethane fraction of FRPA has an enhancement effect on anti-allergic asthma and provide the experimental basis for exploring the processed mechanism of RPA.
登录
查看更多内容
影响因子：
--
作者：
Qu YL;Liu J;Zhang LX;Wu CM;Chu AJ;Wen BL;Ma C;Yan XY;Zhang X;Wang DM;Lv X;Hou SJ
通讯作者：
Hou SJ
影响因子：
5.6
作者：
Wang R;Xiong AZ;Teng ZQ;Yang QW;Shi YH;Yang L
通讯作者：
Yang L
DOI：
10.1038/s41579-020-00461-z
发表时间：
2021-03
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
Vandenberg O;Martiny D;Rochas O;van Belkum A;Kozlakidis Z
通讯作者：
Kozlakidis Z
影响因子：
5.1
作者：
Ma, Baowei;Athari, Seyyed Shamsadin;Zhao, Limin
通讯作者：
Zhao, Limin
影响因子：
5.4
作者：
Tirpude, Narendra Vijay;Sharma, Anamika;Acharya, Vishal
通讯作者：
Acharya, Vishal