突触囊泡的循环对于维持神经突触信号的可靠传递至关重要，是决定突触信息处理容量的重要因素。本课题组曾对calyx of Held突触在室温下从静态开始的囊泡循环的动力学及囊泡循环池的容量进行了定量的分析。最新的研究发现在生理温度下突触囊泡内吞环节的形式、分子机制完全有别于室温条件，挑战了建立在室温条件下观测的囊泡循环动力学及其途径的相关论述，提出了对囊泡循环的动力学再认识的客观要求。本课题拟结合电生理和生物物理学方法，对生理温度下和动态活动条件下calyx of Held突触囊泡循环的过程及其动力学进行完整的定量解析。

Synaptic vesicle recycling is essential for reliable synaptic transmission and largely determine the capacity of information procession at synapse. We have quantitatively analyzed the kinetics of synaptic vesicle recycling from resting status at the Calyx of Held synapse under room temperature. However, the recent discoveies revealed that the cellular and molecular pathway of synaptic vesicle endocytosis under physiological temperature was largely different from what observed under room temperature and thus challenge the knowledge obtained in observation of vesicle recycling under room temperature and called for updated theory of synaptic vesicle recycling. This project is to study the vesicle recycling at the Calyx of Held synapses under physiological temperature with combination of electrophysiology and biophysical modeling and thus to achieve the full description and kinetic dissection of synaptic vesicle recycling at the Calyx of Held synapse.