脉冲电磁场通过激活HuR调控 Sirt1/eNOS 信号通路改善老年鼠血管老化的作用及机制研究

血管老化所致的缺血性疾病严重威害着老年群体的身体健康和生活质量，研究证实脉冲电磁场具有改善内皮细胞功能提高组织供血的作用，但其具体机制尚未完全明了。本课题组前期研究发现，脉冲电磁场能改善内皮细胞功能，且显著上调 RNA 结合蛋白人抗原R ( HuR )的表达，同时发现 Sirt1/eNOS 信号通路的激活，提示脉冲电磁场改善血管老化的功能可能与HuR上调继而影响 Sirt1/eNOS 信号通路有关。本研究拟围绕 Sirt1/eNOS 信号通路相关因子，构建人脐静脉内皮细胞衰老模型和老年小鼠动物模型，运用基因敲除技术，在细胞和动物的水平利用脉冲电磁场干预 HuR 活性，观察 Sirt1/eNOS 信号通路相关因子的表达，明确脉冲电磁场改善老年鼠血管老化的作用及分子机制，为老年缺血性疾病的防治提供新的理论依据和治疗靶点。

Ischemic diseases caused by vascular aging are deteriorating the health and life quality in elder people seriously. Researches have found that pulsed electromagnetic field can improve tissue perfusion by elevating the function of vascular endothelial cells. But the mechanism is still unclear. We have discovered in the previous researches that pulsed electromagnetic field has the ability to elevate the function of vascular endothelial cells, and increase the expression of HuR remarkably. We've also found that Sirt1/eNOS signal pathway was activated during the process, which may have connection with increased HuR and improved vascular endothelial cell function due to the effect of pulsed electromagnetic field. In this research we'd like to focus on exploring the Sirt1/eNOS pathway. My team intend to build models of aged mice and aged vascular endothelial cells from the human umbilical vein. By using the technique of gene knock-out, which allow us to observe on cellular and animal level, we are able to find molecules and correlation factors produced by the activated signal channel Sirt1/eNOS when pulsed electromagnetic field effects HuR. Thus we can clarify the molecular mechanism of pulsed electromagnetic field preventing vascular aging in elder mice. This will provide us with new theory and therapeutic targets for vascular diseases in elder people.