斑茅（Erianthus arundinaceus）是甘蔗重要的近缘属植物，它含有甘蔗育种需要的优良性状。斑茅的利用是甘蔗种质创新的重要内容。但由于甘蔗与斑茅远缘杂交结实率低、F1育性差和杂交授粉生殖发育机理不明确，使得斑茅利用进展缓慢。本项目从细胞生物学角度，利用荧光显微技术和激光共聚焦显微技术等手段，测定父本花粉活性，观察甘蔗与斑茅远缘杂交授粉后花粉萌发、花粉管生长，以及胚胎形成和发育的过程，以探索甘蔗与斑茅远缘杂交不亲和性的机理和甘蔗与斑茅杂交胚胎发育机制，揭示甘蔗与斑茅杂交结实率低的原因，为今后指导甘蔗与斑茅进行合理的杂交，解决杂交瓶颈提供方向，同时将对甘蔗与其它近缘属植物的远缘杂交提供重要的应用参考价值。

E.arundinaceus is an important relative plant of saccharum. It has desirable traits for sugarcane breeding. The Utilization of Erianthus arundinaceum is a major subject for the germplasm innovation of sugarcane. But due to low seed fertility, highly sterile of F1 and unclear mechanism of reproductive development in the distant hybridization between sugarcane and E.arundinaceum, the hybriding utilization of E.arundinaceum makes slow progress in sugarcane breeding. From the perspective of cell biology, the study tested pollen viability of male parent , observed pollen germination and pollen tube growth on the stigmas after pollination, and examined embryo formation and development by using means such as fluorescence microscopy and laser scanning confocal microscopy. It is aim to explore the mechanism of distant cross-incompatibility and embryonic development, and reveal the factors leading to infertility in the crosses between sugarcane and E.arundinaceum, which will instruct reasonable hybridization between sugarcane and Erianthus arundinaceum and provide direction for settling hybridization bottlenecks. At the same time it will provide important reference value for distant hybridization between sugarcane and other relative plants.