肝细胞肝癌依然是世界范围内最常见和致死率最高的肿瘤之一，肝癌的高复发和高转移率是导致肝癌预后不佳的主要原因，有研究显示肿瘤干细胞的存在导致了多种肿瘤的高复发和高转移，寻找肝癌的干细胞相关信号通路有助于肝癌早期诊断和治疗。TGF-β诱导的上皮-间质转化（EMT）同时涉及器官发育、细胞干性、肿瘤细胞转移的调控，是肝癌细胞干性特征的外在表现。本课题组在前期研究中完成了肝癌细胞EMT模型、人胎肝-成肝组织的lncRNA芯片检测，筛选到PVT1、ATB等长链非编码RNA介导了TGF-β调控网络，与肝癌患者预后相关，基于此，本课题拟利用转录因子预测、RIP-seq等技术对上述功能性lncRNA的上、下游调控机制进行深入挖掘，并整合干细胞芯片数据，寻找更多肝癌发生早期的分子事件，完善lncRNA介导的TGF-β调控网络，为肝癌诊断找到更敏感检测指标，为肝癌治疗确定新靶点，以上内容经检索无报道。

At present , hepatocellular carcinoma (HCC) still is one of the most common and the highest death rate cancers all around the world, and the material cause of the poor prognosis of HCC patients is the high rates of recurrence and metastasis of HCC . Some studies show that the existence of cancer stem cells results in the high rates of recurrence and metastasis of many cancers ,so finding the related signal pathway of the stem cell of liver cancer is helpful to the early diagnosis and treatment of HCC patients . TGF-β induced epithelial-mesenchymal transition(EMT), which involves the regulation of organ development, the stem of cell and the metastasis of tumor cells, is the external performance of the stem features of liver cancer. Based on the above analysis, we have completed lncRNA Chip assay of the EMT cells and the human liver tissue. We have screened some long noncoding RNAs , such as PVT1, ATB, NEAT1 and so on , which mediate TGF-βnetwork and are related with the prognosis of HCC patients. On this basis, we plan to use some technologies ,such as transcription factor prediction, RIP-seq, RNA pulldown and so on ,to study deeply the upstream and downstream regulatory mechanisms of the above functional lncRNAs. Furthermore, by integrating stem cell Chip data, we are looking for more the early molecular events of HCC, in order to complete the lncRNA-mediated TGF-β network , find more sensitive detection index, and identify new targets for the treatment of HCC patients. The above content still have not been reported.