近年来干细胞治疗子宫内膜损伤在动物实验和部分临床研究中得到了较好的治疗效果，其中的作用原理和调控机制成为新的研究热点。本课题组在前期研究中建立了多种子宫内膜损伤动物模型，完成了大鼠骨髓间充质干细胞治疗子宫内膜损伤的动物实验，获取了子宫内膜样本并用转录组芯片分析了损伤修复前后的表观遗传学分子改变，在干细胞的外泌体中找到多个可能介导内膜修复的表观遗传分子，基于此，本课题将在后续的研究中以干细胞外泌体为切入点，首先明确其在干细胞促进子宫内膜损伤修复中的作用，然后通过高通量的筛选技术，鉴定出外泌体中调控干细胞促进子宫内膜损伤修复的关键非编码基因，最后以候选非编码基因为线索发现干细胞促进子宫内膜损伤修复过程中关键的表观遗传调控位点（如DNA甲基化区域、组蛋白甲基化和乙酰化位点），为子宫内膜损伤修复药物的研究奠定理论基础。

In recent years, stem cell therapy for endometrial injury has achieved good therapeutic effects in animal experiments and some clinical studies.Physi CAL in prophase study various endometrial injury animal model is established and completed the rat bone marrow mesenchymal stem cells for the treatment of endometrial lesions between animal experiment, obtain the endometrial samples and transcriptome chip damage repair were analyzed before and after the epigenetic molecular changes, outside the stem cells secrete body found in multiple may be mediated lining repair epigenetic molecular, based on this, this topic will be in a follow-up study using stem cells secrete body as the breakthrough point, first clarify its role in stem cells promote restoration of the endometrial lesions, and then by high-throughput screening technology,Identifying the secretion regulation of stem cells in the body outside the key to promote restoration of the endometrial lesions non-coding genes, and finally to the candidate non-coding genes for clues found stem cells promote restoration of the endometrial lesions key epigenetic regulation in the process of loci (such as DNA methylation, histone methylation and acetylation site), for studies of endometrial injury repair drugs to lay the theoretical foundation.