对转基因食品的具体评估，不论是对转基因产品进行标识管理，或是对转基因与非转基因原料的分别输送，还是对转基因原料和食品的检测都是必不可少的。电化学DNA传感器因其响应较快﹑选择性好﹑灵敏度高﹑成本低等优点，已成为生物学、医学领域新课题，其中开发简便、迅速、廉价、微型化的DNA传感器已成为这些领域的研究热点之一。本课题拟利用核酸分子生物识别事件中耦合电化学响应信号变化，结合多途径信号放大策略，实现对食品中转基因植物特征基因序列的直接检测。在前期工作基础上，本课题针对一般基于核酸检测方法需要放大目标分子时存在操作复杂、成本高等不利方面，开展生物功能化纳米材料传感界面上的特征DNA靶标检测研究，建立特异性强、灵敏度高、简单实用的转基因食品特征序列的快速检测技术，研究并构建基于生物功能化碳纳米材料的转基因食品特征序列电化学超灵敏传感机制。本研究将对保障食品的安全性及公众健康具有重要的实际意义。

The specific assessments of genetically modified foods (GMFs) are of great essentials to the identification of genetically modified products management, and the transportation of transgenic and non-transgenic raw materials, as well as the detection of genetically modified raw materials. Electrochemical DNA sensors have become a new research topic in biology and medicine, due to its quick response, good selectivity, high sensitivity and low cost. The development of simple, rapid, cheap, and miniaturized DNA sensor has become a research hotspot. Therefore, it is very important to establish a rapid nucleic acid-based electrochemical sensing method for detection of specific sequences of genetically modified plant components in food. This project is to realize the direct detection of specific sequences of GMFs at rather low concentration, by using the change of electrochemical response signal before and after the recognition events of nucleic acid molecules, combined with multiple signal amplification strategies. On the basis of our preliminary work, the project is to investigate and establish the sensing mechanism for specific genetically modified plant DNA sequences in GMFs, and then develop ultra-sensitive method for specific DNA sequences’ detection in GMFs by caring out the detection on biofunctionalized nanomaterials’ interface. The detection method will possess the advantages of high sensitivity and selectivity, cost-effectiveness, and the portability of instrument, which will overcome the disadvantages of general nucleic acid-based detection methods. The method will show potentials for the increasing demand for further development in the food safety and public health.