糖尿病肾病（DN）是糖尿病常见的慢性微血管并发症，也是引起终末期肾功能衰竭的最重要原因之一。肾脏高表达细胞因子信号传导抑制蛋白-1(SOCS-1)能够减轻糖尿病早期肾脏病变及减轻肾脏的炎症，在DN病变中起到重要的保护作用。荔枝核是治疗DN的传统中药，本项目拟采用建立在体小鼠糖尿病肾病模型和体外诱导肾小球系膜细胞，通过免疫组化、ELISA、Western blot和实时定量PCR等实验方法，从核酸和蛋白两个水平研究荔枝核活性成分荔枝核皂苷影响SOCS-1下调JAK/STAT 信号通路，进而延缓DN进程的作用机制，为临床应用中药防治DN提供理论与实验依据，有助于荔枝核皂苷成为具有自主知识产权的以SOCS-1为靶点的DN治疗药物，具有重要的科学意义和广阔的应用前景。

Diabetic nephropathy is an important microvascular complication of diabetic mellitus as well as the common reason resulting in chronic renal failure. suppressors of cytokine signaling-1 could ameliorate renal pathological changes and Inflammation,and SOCS has protective functions against renal pathological changes. Semen litchi,one of traditional Chinese medicinal, was mainly prescribed in treatment of diabetic nephropathy. This project aims to establish Mouse models of diabetic nephropathy and In vitro activated glomerular mesangial cell model, to study on mechanisms of Influence of saponin of litchi seed on SOCS-1 and Suppress JAK/STAT Pathway to Postpone the process of Diabetic nephropathy through immunohistochemistry, ELISA, Western blot and real-time quantitative PCR and other experimental methods, to provide theoretical and experimental evidence for preventing and treating diabetic nephropathy,and can assist saponin of litchi seed becoming an attractive therapeutic agent with independent intellectual property right for the treatment of fibrotic diabetic nephropathy with SOCS-1 as target.The study would have important scientific significance and application value.