目前研究中对具有抗肿瘤真菌蛋白的临床试验或实际应用的报道还很少，抗肿瘤真菌蛋白及其作用靶点的研究具有广大的开发空间和挖掘潜力。为开发香菇菌C91-3中抗肿瘤蛋白，本课题组对香菇菌C91-3转录组进行了Solexa高通量测序，获得了香菇菌C91-3的转录组基因序列及其功能注释。拟通过筛选，比对及RACE方法获得Latcripin-4基因全长。再经过基因 克隆并诱导高效原核表达系统获得蛋白Latcripin-4，通过鉴定Latcripin-4抗肿瘤活性，以及检测Caspase3、8、9，PI3K，Bcl-2等凋亡相关指标，初步确定Latcripin-4在诱导肿瘤细胞凋亡通路作用中的分子机制；阐述Latcripin-4蛋白诱导细胞凋亡作用的抗肿瘤分子机制，为寻求新的毒副作用小、靶向作用强的肿瘤治疗蛋白药物开辟新的途径。

Cancer has become the most serious disease threatening the health of human beings. the anti-tumor fungal proteins have showed their great effect and potential, but the study of the anti-tumor fungal protein rarely reported in clinical trials or the practical application. To identify and develop the anti-tumor protein in Lentinula edodes C91-3, we conducted a high-throughput transcriptome sequencing experiment to obtain Lentinula edodes C91-3 transcriptome sequences. Through a comprehensive bioinformatics analysis, we preliminarily screened the transcriptome sequence which is correlated to antitumor activity . According to the transcriptome sequence, the full-length gene of Latcripin-4 was obtained by 3',5'-Full RACE (Rapid Amplificatioon of cDNA Ends) method. We induced and expressed the protein Latcripin-4 with Prokaryotic expression system. Through identifying anti-tumor activity of Latcripin-4 and detecting the indexes(Caspase3、 8、9, PI3K, Bcl-2), the molecular mechanism will be initially identified in the apoptosis pathway which Latcripin-4 induced the tumor cell.Finally, we try to illuminate the molecular mechanisms of Latcripin-4 protein on inducing cancer cell apoptosis, which will bring new insights and advantages in founding effective anti-tumor protein medicine.