In vitro antitumor activity of Latcripin-15 regulator of chromosome condensation 1 domain protein.
In vitro antitumor activity of Latcripin-15 regulator of chromosome condensation 1 domain protein.
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Latcripin-15 染色体浓缩 1 结构域调节蛋白的体外抗肿瘤活性。
DOI：
10.3892/ol.2016.5106
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发表时间：
2016-11
期刊：
影响因子：
2.9
通讯作者：
Zhong M
中科院分区：
文献类型：
--
作者：
Tian L;Wang X;Li X;Liu B;Zhang W;Cao J;Ning A;Huang M;Zhong M
癌症是世界范围内最重要的健康问题之一，因此需要开发具有较少副作用的新型治疗剂。本研究探讨了一种新分离的真菌蛋白的体外抗癌作用。本研究从香菇C91-3菌株中分离到一个染色体凝集1（RCC 1）结构域蛋白Latcripin-15（LP-15），并对其进行鉴定、克隆、表达、纯化和复性，以评价其体外抗肿瘤活性。采用3′和5′末端快速扩增法从香菇中分离到LP-15 RCC 1全长cDNA，并进行克隆、表达、纯化和复性。此外，通过3-（4，5-二甲基噻唑-2-基）-2，5-二苯基四氮唑溴化物测定、透射电镜、流式细胞术和Hoechst 33258染色来评估分离的LP-15 RCC 1蛋白的功能结构域对人肺癌A549细胞的存活和凋亡的影响。成功表达、纯化并复性了LP-15 RCC 1功能域蛋白。用LP-15 RCC 1功能结构域蛋白处理显著降低肿瘤细胞活力并诱导A549细胞凋亡。本研究的结果表明，LP-15 RCC 1功能结构域需要进一步研究作为一种新的治疗药物用于癌症治疗。
Cancer is one of the most significant health problems worldwide and thus the development of novel therapeutic agents with fewer side effects is required. The present study investigated the in vitro anticancer effects of a newly isolated fungal protein. In this study, Latcripin-15 (LP-15) regulator of chromosome condensation 1 (RCC1) domain protein, which is obtained from the Lentinula edodes C91-3 fungal strain, was identified, cloned, expressed, purified and re-folded to assess the in vitro antitumor activity of the protein. LP-15 RCC1 full-length cDNA was isolated from Lentinula edodes using 3′ and 5′-rapid amplification of cDNA ends and then cloned, expressed, purified and re-folded in vitro. In addition, the effects of the isolated LP-15 RCC1 protein's functional domain on the viability and apoptosis of human lung cancer A549 cells were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, transmission electron microscopy, flow cytometry and Hoechst 33258 staining. The LP-15 RCC1 functional domain protein was successfully expressed, purified and re-folded in vitro. Treatment with the LP-15 RCC1 functional domain protein significantly reduced tumor cell viability and induced apoptosis in A549 cells. The results of the present study indicate that the LP-15 RCC1 functional domain requires further investigation as a novel therapeutic agent for cancer therapy.
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DOI：
10.1016/j.ijbiomac.2012.10.007
发表时间：
2013-01-01
影响因子：
8.2
作者：
Jeff, Iteku Bekomo;Yuan, Xiaowen;Zhou, Yifa
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