基于微流控芯片技术构建PLGA/Chitosan复合载药神经导管及在周围神经损伤修复中的应用研究

周围神经损伤是世界性的医学难题之一。申请人团队的前期研究发现壳聚糖神经导管联合外科手术用于周围神经损伤修复具有很好的疗效。随着交叉学科的迅速发展，载药缓释组织工程导管有望在神经损伤研究中取得新的突破。但目前该领域仍有许多问题函待解决：导管负载药物的配比和浓度如何筛选？导管载药缓释时机如何做到与体内需求一致？单一材料神经导管的性能局限如何突破？复杂神经损伤修复能力如何进一步提升？等。为进一步促进载药缓释神经导管的相关研究，申请者拟开展：1）基于微流控芯片技术，体外仿生构建周围神经微器官，筛选神经因子促进神经再生的适合浓度与配比；2）构建和优化个性化载神经因子的人工材料（PLGA）/生物材料（Chitosan）复合载药神经导管，并在周围神经损伤动物模型中进行应用并探讨相关机制。本研究面向临床急需解决的重要问题，拟通过建立和优化个性化的复合材料载药缓释神经导管，来加速周围神经损伤的治疗和康复。

Peripheral nerve injury is one of the most difficult medical problems in the world. In recent years, a large number of studies proved that neural tube combined with neurosurgery had a good effect for the repair of peripheral nerve injury. We have independently invented an absorbable chitosan neural tube and used successfully in animal and clinical multicenter studies, which promoted Chinese study about repair of peripheral nerve injury in the leading position of the world. In order to further promote the treatment of peripheral nerve injury, we will conduct the following studies: 1) establish an individual neural drug screening method based on the biomimetic microfluidic technology; 2) develop a multi drug loading artificial material (PLGA) and biomaterial material (chitosan) composite nanoscale neural tube; and further study the multiple regeneration and repair of peripheral nerve injury by the new innovative neural tube. This study is geared to the major clinical problems, and we wish to establish a new nerve repair method by the establishment of individual drug loading PLGA/chitosan neural tube combined with multiple regeneration and repair theroy.