金黄色葡萄球菌（Staphylococcus aureus ,S.aureus）乳腺炎是影响畜牧业发展的主要疾病之一。目前，金黄色葡萄球菌乳腺炎感染的相关调控机制并不完全清楚， 深入研究其免疫机制已迫在眉睫。近年来研究表明，γδ T 细胞在细菌感染和慢性炎症中发挥重要作用。尤其是最近研究发现，γδ T 细胞及其分泌因子 IL-17在 S.aureus皮肤感染中发挥了重要的免疫保护作用。乳腺作为机体的特殊组织器官，在感染金黄色葡萄球菌后其γδ T 细胞及其主要细胞因子动态变化如何？发挥怎样的功能？目前还鲜有报道。本研究将从金黄色葡萄球菌小鼠乳腺炎和奶山羊乳腺炎两种模型入手，全面系统分析γδ T细胞在乳腺感染S.aureus中的动态变化规律、所发挥的功能和调节机制，从而为阐明金黄色葡萄球菌乳腺炎感染的免疫机制和疫苗研制提供新的实验证据。

Staphylococcus aureus mastitis is the most common and costly livestock diseases. The mechanism of persistent infection of mammary gland by Staphylococcus aureus was complex and need to be understood urgently. Several Literatures indicated γδ T cell is responsible for both protection and chronic status of certain infections, and recent findings highlight an important role for IL-17 γδ T cells in clearance of S. aureus in mouse skin providing a new target for the prevention and treatment of Staphylococcus aureus skin infections. However, the role of γδ T cell during mammary gland Staphylococcus aureus infection is unknown. In this study, we will investigate characteristics and the role of γδ T cell against Staphylococcus aureus induced mastitis in mouse and dairy goat models .This study will lay the basis to understand the role of γδ T cell in the pathogenesis of Staphylococcus aureus mastitis.