Z-DNA 构型在DNA 转录，染色质重塑和DNA 重组等过程中都扮演着重要角色。但实验识别Z-DNA构型十分困难，而目前又缺乏全基因组范围内识别Z-DNA 形成序列的可靠生物信息学工具。也缺乏对基因组各功能区Z-DNA 形成序列分布的系统分析。我们将结合DNA 序列片段碱基构成和B-Z 构型转化所需能量，以DNA 负超螺旋密度为标准，开发全基因组范围识别Z-DNA 形成序列的新方法，并通过Z-DNA 结合蛋白域Zalpha-ADAR1 与Z-DNA构型的染色质亲和沉淀实验（Chromatin Affinity Precipitation）的现有实验数据评估我们的Z-DNA 识别方法，优化关键参数， 编制相应的计算机程序，预测并分析基因组各功能区域Z-DNA 形成序列的分布。

Z-DNA conformation was reported to play important roles in DNA tricrption, chromation remodeling and DNA recombination. However, it is very difficult to experimentally identify Z-DNA segments in the human genome, and to date it is lack of reliable bioinformatic tools to conduct whole genome wide Z-DNA scanning. Therefore, there is no systematic study on the distribution of Z-DNA over different fuctional regions in the genome. We propose here to develop a novel bioinformatics approach to identifying Z-DNA forming sequences at whole genome level by combining the information of DNA base compostion and the energy requirment of a DNA sequence to undergo a B-Z transition, and the energy requiement will be calculated in terms of negative supercoiling density. We will use the current available data of chromatin affinity precipitation (CHAP) experiments of the Z-DNA binding domain Zalpha-ADAR1 and the Z-DNA conformation to evaluate our Z-DNA scanning method and to optimize the key parameters. The developed Z-DNA scanning tool will be finally used to analyze the Z-DNA distribution over various functional regions in the human genome.