本项目拟基于申请者病毒学和分子生物学专业背景，以及在荧光纳米材料的设计、构建以及生物应用方面的研究经历，设计合成具有特异性结合重组病毒表面膜蛋白能力的荧光锁频纳米粒子，并通过与经基因工程改造，带上绿色荧光蛋白标记的细胞表面病毒受体蛋白相互作用，获得一组可以相互作用的生物大分子和荧光纳米粒子复合体系。该体系中病毒膜蛋白与受体之间的相互作用可以通过可调制的荧光共振能量转移现象加以分析。同时由于本研究合成纳米粒子的荧光锁频特性，可以消除系统中背景荧光的干扰，使这一体系可以应用于从富含荧光背景的中草药粗提物中直接筛选病毒受体结合抑制剂。在无病毒受体结合抑制剂存在时，该体系显示强荧光调制效应，而在有病毒受体结合抑制剂存在时，荧光调制效应降低或消失。该新体系也可用于研究已知有抗病毒效果中草药的抗病毒机理和抗病毒靶点的确定。

Based on the applicant's virology and molecular biology background, as well as experiences in the design, construction and investigation on the biological applications of fluorescent nanomaterials, this project aims to design and synthesis a frequency-locked fluorescent nanoparticle which can specifically bind the recombinant virus membrane protein. And through binding the green fluorescent protein labeled cell surface receptor, the nanoparticles-virus protein-receptor will form a complex, by which their interactions can be investigated using modulatable fluorescence resonance energy transfer (FRET) analysis. The frequency-locked characteristics of the fluorescent nanoparticle can help to eliminate the background interference fluorescence in the drug screening system. This novel system can be used in directly screening virus-receptor inhibitors from the crude extraction of Chinese herbal medicine, which has lots of background fluorophore. When the screening system has no virus-receptor inhibitor inside, a strong modulation effect will be detected, while the virus-receptor inhibitor exists, the modulation effect will be decreased or disappear. This system can also be used to study the antiviral mechanism and antiviral target of Chinese herbal medicine which has known antiviral activities.