肿瘤治疗面临的最大瓶颈之一是肿瘤复发和转移，其真正原因可能是肿瘤干细胞（CSC），以CSC为靶点寻找新的肿瘤治疗方法，具有重要临床意义。根据最新CSC研究成果和本课题组前期研究基础，本项目拟进行以下研究：（1）通过构建CD44、HER2高表达鼻咽癌细胞模型，CD44、HER2基因敲除鼻咽癌CSC模型以及其动物模型，深入研究鼻咽癌CSC Ras信号通路特征及CD44对该信号通路的调控作用；（2）观察益气解毒颗粒联合盐霉素对体内外鼻咽癌CSC增殖、凋亡、侵袭与转移的影响；（3）通过检测CD44、HER2、Ras信号通路蛋白及其下游效应蛋白表达水平和活性，从CD44/Ras信号通路深入研究益气解毒颗粒与盐霉素联合作用分子机制。该研究可为鼻咽癌治疗靶点药物开发提供理论依据；为益气解毒颗粒广泛应用奠定基础；为中西医联合用药协同增效提供科学依据；从肿瘤干细胞研究中西医抗癌作用，为肿瘤防治开辟新途径。

One of the biggest problems in cancer treatment is tumor recurrence and metastasis, which may be related with cancer stem cells (CSC).Targeting CSC to find new methods of cancer treatment has important clinical significance. According to the latest CSC research results and preliminary research results of our group, the project intends to carry out at three aspects: (1) Ras signaling pathway characteristics in nasopharyngeal carcinoma(NPC) CSC and the regulation of CD44 on the signaling pathway will be studied deeply by building CD44, HER2 overexpression nasopharyngeal carcinoma cell model, CD44, HER2 gene knockout nasopharyngeal CSC model and its animal model; (2) Effects of Yiqi Jiedu granule combined with salinomycin on NPC CSC proliferation, apoptosis, invasion and metastasis in vivo and in vitro will be observed; (3) Molecular mechanisms of Yiqi Jiedu granule combined with salinomycin will be studied further from the CD44 / Ras signaling pathway by detecting the protein expression levels and activity of CD44, HER2, Ras signaling pathway and their downstream effector proteins. The research can provide theoretical basis for the new targeting drug development of the treatment of nasopharyngeal carcinoma，is also the further clarification of the role of Yiqi Jiedu granule and can lay the foundation for its wide applications. It provides scientific basis for synergies effects of the combination of Chinese and Western medicines, and offers a new way for anticancer.