果实成熟是一个非常复杂且受多个转录因子精细调控的动态过程，其中番茄RIN通过转录因子复合体激活大量成熟基因的表达，是果实成熟的关键调控因子。我们前期的研究发现番茄RIN转录因子对果实过度成熟起抑制作用，但具体机制不清楚。本项目拟通过酵母双杂（Y2H）和免疫沉淀-质谱（IP-MS）技术鉴定RIN的互作蛋白，进一步通过蛋白结构域分析、亚细胞定位、蛋白互作验证、转录调控和启动子结合分析，解析RIN转录因子复合体对果实过度成熟相关靶基因的调控模式，明确RIN复合体抑制果实过度成熟的分子机制，为完善转录因子调控果实成熟的生物学理论，并为培育良好性状的番茄果实提供新的思路。

Tomato fruit ripening is a complex developmental process, which is controlled by multiple transcription factors. RIN is a key regulator in fruit ripening, which forms transcription factors complex and activates the expression of multiple ripening related genes. Our previous study proved that RIN inhibit the over-ripening in fruit, however, its mechanism remains unclear. The present program will focus on identification and analysis of RIN-interacting factors in tomato fruit by yeast-two-hybrid(Y2H) and Immunoprecipitation mass spectrometry (IP-MS). Using protein domain analysis, subcellular localization, protein interaction assay, trans-activation and promoter binding assays, it will clarify RIN complex in regulating the expression of target tomato over-ripening genes, then identify the mechanism of RIN complex inhibit progress of over-ripening. Improved understanding of this transcription factor regulation will make it possible to alter the regulation of tomato target ripening genes in order to produce high quality tomato fruit.