程序性细胞死亡（PCD）是多细胞真核生物最重要的生物学过程之一。PCD在植物的生长发育和逆境响应中发挥着至关重要的作用，但植物调控PCD的分子机制还很不清楚。类病斑突变体是解析植物PCD分子机理的有效工具。在前期研究中，我们通过筛选拟南芥类病斑突变体的抑制子克隆到一个正调控PCD的基因SOL1。SOL1含有蛋白磷酸酶结构域，定位于细胞核中，其生物学功能尚无报道。本项目将结合遗传学、蛋白质组学、生物化学和分子生物学等手段进一步研究SOL1调控PCD的机理，鉴定SOL1上游或者下游的信号组分。本项目的开展有助于发现植物调控PCD的新组分（SOL1及其互作蛋白）和新机制，具有重要的科学意义。

Programmed cell death (PCD) is one of the most important biological processes in multicellular eukaryotes. PCD plays an essential role in plant growth, development and stress responses. However, the molecular mechanisms of PCD in plants remain largely unknown. Lesion-mimic mutant is a powerful tool to decipher how plants regulate the PCD. In previous studies, we identified a gene named SOL1 that positively regulated PCD through screening for the suppressors of an Arabidopsis lesion-mimic mutant. SOL1 contains a protein phosphatase domain and is localized in the nucleus, and its biological functions have not been reported yet. In this project, we will further study how SOL1 regulates PCD and identify its upstream or downstream components using integrated approaches of genetics, proteomics, biochemistry, and molecular biology. This proposal will help to discover new components (SOL1 and its interacting proteins) and new mechanisms of PCD in plants, which is of important scientific significance.