植物再生是植物体对受损结构自我修复或替代的过程。再生能力不仅是植物发育可塑性的根本，也是现代植物生物技术的基础。年龄是控制植物再生能力的关键因素，尤其多年生的木本植物，随着其年龄的增加，再生能力明显逐渐降低，但是年龄调控木本植物再生能力的分子机制尚不清楚。已有研究表明，年龄通过影响生长素的合成调控了拟南芥不定根从头再生，且本项目已有的研究基础暗示，生长素在杨树不定根从头再生过程中发挥着重要作用。据此，本项目推测年龄途径可能位于生长素行为的上游调控木本植物的不定根再生。本项目将综合运用高通量测序，遗传学，分子生物学及细胞学等方法，以生长素行为为线索，借助模拟自然条件的杨树不定根从头再生体系，挖掘木本植物年龄的响应因子，阐明年龄调控杨树不定根从头再生的分子机制，推动林木快繁技术和林业遗传改良工程的发展。

Plant regeneration is the process, in which the plant body repairs or replaces damaged tissues or organs. The regeneration ability is not only the root of plant development plasticity, but also the basis of modern plant biotechnology. Age is the critical factor in controlling the regenerative ability, especially for the perennial woody plants. With the increase of their age, the regeneration ability decreases gradually. However, the molecular mechanism of age regulating the regeneration ability of woody plants remains poorly understood. Previous studies have shown that age regulates adventitious root regeneration by influencing the synthesis of auxin in Arabidopsis, and the existing research basis of this project suggests that auxin plays an important role in the process of adventitious root regeneration in poplar. Based on this, the project speculates that the age pathway may be located upstream of auxin behavior to regulate the adventitious root regeneration of woody plants.This project will use sequencing, genetics, molecular biology, and cytology methods, and take auxin behavior as a clue. In addition, research system is based on adventitious root system of poplar that simulates natural conditions. The purpose of this project is to findout the response factors of age in woody plants, clarify the molecular mechanism of age regulating the regeneration ability of adventitious root in poplar, and then promotes the development of rapid tree propagation technology and forestry genetic improvement projects.