化瘀通络法常用于治疗缺血性脑卒中，且具有确切临床疗效，尤其在神经功能损伤恢复上。鉴于化瘀通络相关研究多集中在血管系统角度并已取得一定研究成果，但对神经元保护研究尚缺乏深入机制探讨，难以全面阐明化瘀通络科学内涵。前期研究采用临床疗效确切的化瘀通络方丹红注射液为探针，通过高通量蛋白质组学等方法，筛选出了与缺血性脑卒中病理过程密切相关的特殊富含AT序列结合蛋白（SATB1）。据此新发现，继续深入研究其在缺血性脑卒中病理过程神经保护所扮演角色，对于进一步提高对缺血性脑卒中的病理认识，并为发现治疗新靶点、从神经元层面阐明“化瘀通络”新的科学内涵尤为必要。本项目拟建立体内小鼠MCAO模型和体外神经元细胞OGD模型，从动物、细胞、分子水平围绕上述科学问题而开展研究，其不仅有助于发现缺血性脑卒中干预的新机制，也有利于阐明“化瘀通络” 神经元层面的科学内涵。

Blood-stasis-removing and collateral-activating therapies is commonly used in the treatment of ischemic stroke, and has definite clinical efficacy, especially in the recovery of neurological damage. In view of the fact that the research related to blood-stasis-removing and collateral-activating therapies focuses on the vascular system and has achieved certain research results, there is still no in-depth mechanism for the research of neuron protection, and it is difficult to fully clarify the scientific connotation of blood-stasis-removing and collateral-activating therapies. In the previous study, Danhong injection, which was the representative prescription of blood-stasis-removing and collateral-activating therapies, was used as a probe with high clinical efficacy. High-throughput proteomics and other methods were used to screen out special AT-sequence-binding proteins (SATB1) that are closely related to the pathological process of ischemic stroke . Based on this new discovery, continue to further study its role in neuroprotection in the pathological process of ischemic stroke, to further improve the pathological understanding of ischemic stroke, and to clarify from the neuron level for the discovery of new therapeutic targets. The new scientific connotation of "blood-stasis-removing and collateral-activating therapies" is especially necessary. This project intends to establish an in vivo mouse MCAO model and an in vitro neuronal cell OGD model, and conduct research on the above scientific issues from the animal, cellular, and molecular levels, which not only helps to discover new mechanisms for ischemic stroke intervention, but also it is helpful to clarify the scientific connotation of "blood-stasis-removing and collateral-activating therapies" at neuron level.