蓝舌病病毒(BTV)感染引起的蓝舌病是一种侵害反刍动物的烈性传染病。BTV新血清型毒株的不断出现，不同血清型毒株之间通过基因重配产生变异毒株，给本病的防控带来了严峻的挑战。2016年，我们在广西分离到一株新血清型BTV毒株，在完成该毒株全基因组测序的基础上，本项目拟进一步在细胞与易感动物两个层面进行新血清型BTV毒株感染与基因重配特性的研究：（1）将病毒分别感染不同物种来源的细胞，在细胞上明确病毒的感染与增殖特性；将病毒分别感染感染绵羊与山羊，在易感动物上明确病毒的感染特性、致病性以及感染动物的免疫应答特征。（2）将病毒与我国分布广泛的BTV-1型毒株分别共感染细胞与易感动物，在细胞与易感动物上分析两种血清型毒株间能否发生基因重配及基因重配频率特征。项目研究结果不仅有助于揭示新血清型BTV在感染特性与基因重配等方面的机制特征，也可为我国蓝舌病的防控提供详实可靠的科学依据。

Bluetongue virus (BTV) is the causative agent of bluetongue, one of the major hemorrhagic diseases in ruminants. Continuous emergence of new BTV serotypes and mutant strains produced by reassortment between different serotypes have brought severe challenge to the prevention and control of Bluetongue. In 2016, a candidate new serotype of BTV was isolated in Guangxi Province. Based upon obtained genome sequence of this new serotype BTV strain, we will further carry out research on infection and genetic reassortment characteristics of this virus in cells and susceptible animals levels. (I) Identify the infection and proliferation characteristics of the virus in diverse cell lines from different species; Demonstrate the characteristics of infection and pathogenicity of the virus and the immune response in artificially infected sheep and goat. (II) After co-infected cells and susceptible animals with the new serotype BTV strain and BTV-1 strain widely distributed in China, analyze whether reassortment could happened between two serotypes of strains and frequency of reassortment. The results of this study will help to reveal the characteristics of the new BTV serotype in terms of infection and genetic reassortment, and also provide a scientific basis for the prevention and control of bluetongue in China.