喉咽癌早期患者生存率明显高于晚期患者，有效微创性的早期诊断在临床上意义重大。肿瘤细胞能分泌大量含有lncRNA的外泌体到血循环中，检测异常lncRNA分子为肿瘤早期诊断提供了新机遇。我们前期研究发现lncRNA PEG10在喉咽癌组织及血清外泌体中均高表达，并与上皮间质细胞转化（EMT）过程关系密切。但lncRNA PEG10与喉咽癌发生的关系以及是否可作为分子标志物用于肿瘤早期诊断尚无研究。本课题拟通过建立细胞EMT模型、小鼠成瘤模型，研究lncRNA PEG10在喉咽癌发生发展过程中的作用机制，同时运用qRT-PCR技术对喉咽癌、癌前病变以及健康对照者血清进行大样本、多层次验证，评价lncRNA PEG10作为分子标记物应用于喉咽癌早期诊断价值。本研究将有助于明确 lncRNA PEG10在喉咽癌发生发展中的作用，也将为喉咽癌的实验室诊断提供依据。

Survival rate for patients with early-stage hopharyngeal carcinoma is higher than that for those with late-stage carcinoma obviously. There is an important significance to diagnose hopharyngeal carcinoma early and mini-invasively in clinical. Tumor cells can release exosomes that contain lncRNAs into the blood. Investigation of aberrant circulating exosomal lncRNAs may provide new opportunity to discover tumor-related biomarkers. In our previous studies, high-throughput lncRNAs chip analysis was performed in tissue samples of hopharyngeal Survival rate for patients with early-stage hopharyngeal carcinoma is higher than that for those with late-stage carcinoma obviously. There is an important significance to diagnose hopharyngeal carcinoma early and mini-invasively in clinical. Tumor cells can release exosomes that contain lncRNAs into the blood. Investigation of aberrant circulating exosomal lncRNAs may provide new opportunity to find tumor-related biomarkers. In our previous studies, we found that the expression of lncRNA PEG10 was high in both tumor tissue and sera exosomes, which was related to the courses of EMT closely. The relationship between lncRNA PEG10 and tumorigenesis is not yet clear. Whether it can be used as a molecular marker for early diagnosis of tumors has not been studied. In this study, we intend to investigate the role of lncRNA PEG10 on the occurrence and development of hopharyngeal carcinoma by using cell EMT model and the vivo nude mice experiments. At the same time, we will further assess the diagnostic values of lncRNA PEG10 by RT-qPCR in large serum samples. It will be helpful to definite the function of lncRNA PEG10 in the course of occurrence and development of cancer, providing new ideas for the study of the pathogenesis and new targets for clinical diagnosis.