弹状病毒属于单链负义RNA病毒，能够侵染脊椎动物、无脊椎动物以及植物等寄主。由于植物弹状病毒反向遗传学体系的缺乏，植物弹状病毒在寄主植物或介体昆虫体内的致病机制研究比较滞后。大麦黄条点花叶病毒(Barley yellow striate mosaic virus, BYSMV)是一种世界范围内广泛分布的植物细胞质弹状病毒，近期在我国北方小麦产区检测到该病毒病害的发生。本研究的前期工作首次报道BYSMV的全长基因组序列，并初步建立了病毒的微型复制子体系。在此基础上，本项目拟针对BYSMV的关键致病因子P蛋白的致病机制展开深入研究，分别从亚细胞定位和磷酸化氨基酸位点入手，逐步分析这些特征与寄主因子的互作关系，解析P蛋白在病毒致病过程中的关键作用，为抗病毒育种提供更精准的分子靶标，并为病毒病害的科学防治提供理论依据。

Rhabdoviruses comprises diverse members of negative-sense, single-stranded RNA viruses with broad host ranges including vertebrates, invertebrates and plants. The pathogenesis of plant rhabdoviruses are largely unknown due to the lack of infectious cDNA clones of plant rhabdoviruses. Barley yellow striate mosaic virus (BYSMV) is a world-wide cytorhabdovirus, and was recently reported to cause disease in the wheat field of North China. In our previous studies, the complete genome of BYSMV was obtained, and the minireplicon systems was successfully established. Based on these results, this proposal will focus on the pathogenic mechanisms of BYSMV P protein, a key determint factor of viral infection, through investigating the functional roles of its subcellular localization and phosphorylation modification, as well as the host-targeting proteins of BYSMV P. These results will provide new insights into understanding of the main functions of BYSMV P acting as accurate molecular targets for antiviral breeding, providing scientific bases for virus disease control.