“肝开窍于目”是中医最重要理论学说之一，认为肝与目的功能状态直接相联。葡萄膜炎是常见、反复发作的致盲性眼病，自身免疫的紊乱是其发病的重要原因。肝郁证是肝病症候的核心，可影响多种自身免疫病的发展与转归，而从中医证候学角度研究肝郁证对葡萄膜炎影响的研究尚属空白。Treg/Th17细胞是自身免疫性葡萄膜炎致病过程中主要的免疫细胞，正常情况下两者处于一种效应平衡状态，两者的失衡对其发病与转归具有重要影响。本课题在中医肝郁证基础上，建立肝郁型EAU病证结合模型，采用行为学观察及单胺类神经递质检测对模型进行评价，并采用流式细胞术、荧光定量PCR、蛋白免疫印迹法、酶联免疫吸附试验等方法，探讨“肝郁”对EAU大鼠的发病及Treg/Th17细胞表达的影响，阐释“肝郁”对EAU影响的分子机制，为深入认识“肝开窍于目”理论奠定基础。

The theory that “liver opening at eye” is one of the most important theories of traditional Chinese medicine, which is usually thought that the eye is directly related to the function of liver. Uveitis, a common cause of vision loss and autoimmune disorder is the major cause for the occurrence of uveitis. The syndrome of “liver depression” plays a central role in liver syndrome, which can affect the development and favorable recovery of multiple liver diseases. However, it is still unknown that how the syndrome of liver depression affects the development of autoimmune uveitis. A number of groups have recently shown that both T regulatory cells (Treg) and T helper cells (Th17) play an important role in the development and maintenance of EAU. However, the out-of-balance between Treg and Th17 will influence the occurrence and recurrence of autoimmune uveitis. Based on the theory of the syndrome of liver depression, an experimental autoimmune rat model with the syndrome of liver depression will be set up, and the model will be further evaluated by using behaviors and the determination of monoamine neurotransmitters. In the meantime, the effects of the syndrome of liver depression on the occurrence of EAU and on the ratio of Treg/Th17 cells in rats will be further investigated by means of flow cytometry, real-time quantitative PCR, western blot, ELISA techniques. Based on those results, the mechanisms of the effect of the syndrome of liver depression on the occurrence and development of EAU will be elucidated, and it will provide the thought and support for the understanding of the theory that “liver opening at eye”.