宿主因子影响血吸虫的生长发育、营养代谢、免疫逃避等诸方面。本研究以国家自然科学基金项目(No:30771876)资助下建立的日本血吸虫肺期童虫与宿主细胞共培养体系及筛选出的与宿主相关差异基因为基础，重点研究其中的钙连蛋白(Canx)、腺苷激酶1(AK1)、生长激素诱导的跨膜蛋白(GHITM)、程序性死亡蛋白10(PCDP10)基因表达产物在血吸虫童虫中的分布及其对生长发育的影响；酪蛋白激酶(CK)基因功能及其产物与宿主细胞的相互作用；组织蛋白酶L(CL)和3-磷酸甘油醛脱氢酶(GAPDH)基因表达及其在逃避宿主免疫过程中的作用，揭示日本血吸虫童虫的生长发育机制及其与宿主的相互关系，阐明肺期童虫免疫逃避的分子机制，为血吸虫病诊断、抗虫药物的筛选与疫苗研究提供新线索，为共培养体系在血吸虫分子生物学、基因功能及其与宿主的相互关系等研究中的应用提供科学依据，开创血吸虫病研究的新局面，具有重要意义。

Host factors were considered to affect the growth and development, nutrition and metabolism, immune escape from host's attack of schistosome and so on. This project, bases on the Schistosoma japonicum schistosomula and host cells co-culture system and the differentially-expressed genes identified using the system which had been successfully established in our last finished NSFC project(30771876), intends to explore the functions of calnexin (Canx), adenosine kinase 1 (AK1), growth hormone inducible transmembrane protein (GHITM) and programmed cell death protein 10 (PCDP10) in the growth and development of schistosomula, and the function of casein kinase (CK) in the interaction between schistosome and the host cells, and the functions of cathepsin L (CL) and glyceraldehydes 3-phosphate dehydrogenase (GAPDH) in the schistosome's escape from host's immune attack.This project aims to interpret the mecular mechanism of schistosme development and their relationship with their host, and to clarify the mecular mechanism of schistosomula's immune evasion, which will provide new clues for schistosomiasis diagnosis, development of antischistosomal drugs and vaccines, and provide scientific basis for application of the co-culture system in researches of molecular biology, gene functions and the relationship between schistosome and their hosts, to create a new area of schistosomiasis research.