慢性前列腺炎/慢性骨盆疼痛综合征（CP/CPPS）是泌尿外科常见的顽固性病理性疼痛综合征，由反复炎性刺激及其他多种因素共同参与形成，现有的镇痛方法效果不佳。最近研究表明激活BNP/NPR-A/PKG/BKCa信号通路可以抑制炎性疼痛大鼠DRG中C-痛觉神经元的兴奋性。本研究小组针对CP/CPPS，建立CP/CPPS大鼠模型，探讨：1、DRG中BNP及NPR-A的表达变化、PKG及BKCa的表达变化及磷酸化变化；2、DRG中C-痛觉神经元的膜电特性,观察其兴奋性变化；3、BNP、NPR-A抑制剂及PKG抑制剂对C-痛觉神经元BKCa通道上钾电流的影响；4、脊髓胶质细胞的活性，分析BNP信号通路缓解CP/CPPS的效果。通过上述研究明确BNP信号通路在CP/CPPS中的作用。本项目深入研究后，若能达到预期目的，将为CP/CPPS找到新的疼痛机制，提供一种全新的镇痛靶标，建立一种全新的镇痛策略。

Chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) is a kind of common and refractory pathological pain syndrome of urinary surgery. It is formed by repeated inflammatory stimuli and many other factors, while existing methods of analgesia are not optimal. Recent studies have shown that activation of BNP/NPR-A/PKG/BKCa signaling pathway can inhibit excitability of C-pain neurons of DRG in inflammatory pain rat. Specific to CP/CPPS, our research group established CP/CPPS rat model, afterwards, 1. To investigate the expression change of BNP and its receptor NPR-A in DRG and to probe the expression change and the phosphorylation change of PKG and BKCa in DRG,2. To probe the change of membrane electrical characteristics and excitability of C-pain neurons,3. To investigate the effect of BNP, NPR-A inhibitor and PKG inhibitor on the amount of potassium current which travel through the BKCa channel of C-pain neurons in DRG,4. To discuss the activity of the spinal cord glia and analyze the effect of the BNP signaling pathway in CP/CPPS. After deep research, we tend to identify the roles of the BNP signaling pathway in CP/CPPS.If expecting goals are achieved, we will find a new pain mechanism of CP/CPPS and provide a firenew analgesic target and analgesic strategy.