S1P/S1PR1/IL-1β/BNP信号通路在慢性前列腺炎/慢性骨盆疼痛综合征 （CP/CPPS）中的作用及机制研究

慢性前列腺炎/慢性骨盆疼痛综合征（CP/CPPS）是泌尿外科常见的顽固性病理性疼痛综合征，由反复炎性刺激及其他多种因素共同参与形成，现有的镇痛方法效果不佳。前期研究发现，BNP在CP/CPPS疼痛的发生、发展中发挥重要作用。此外，S1P、S1PR1、IL-1β在大鼠CP/CPPS中显著上调中，且与BNP呈正相关，S1PR1的下调可缓解CP/CPPS疼痛。综合目前已有研究、前期研究基础及预实验结果，我们推测S1P/S1PR1 /IL-1β/BNP信号通路参与CP/CPPS的发生、发展。本项目在已有研究的基础上，我们采用基因工程、脊髓鞘内注射、免疫荧光等技术，在CP/CPPS大鼠体内进一步深入研究S1P/S1PR1/IL-1β/BNP在CP/CPPS中的重要作用，以期为 CP/CPPS找到新的疼痛机制，提供一种全新的镇痛靶标。

Chronic prostatitis / chronic pelvic pain syndrome (CP / CPPS) is a common refractory pathological pain syndrome，It is formed by repeated inflammatory stimulation and other factors. At present, there is no effective treatment. In the previous study, BNP play an important role in the occurrence and development of CP / CPPS . In addition, S1P, S1PR1 and IL-1 β were significantly higher expression in CP / CPPS and positively correlated with BNP. Furthermore,The inhibition of S1PR1 could relieve the pain of CP / CPPS. Based on the previous research, we proposed that S1P / S1PR1 / IL-1 β / BNP signaling pathway is involved in the occurrence and development of CP / CPPS. we adopted gene engineering, intrathecal injection of spinal cord and immunofluorescence and so on to further study the important role of S1P / S1PR1 / IL-1 β / BNP in CP / CPPS . To find a new pain mechanism of CP / CPPS and provide a new analgesic target