驯化土著微生物菌群原位修复PAHs污染土壤的规律及机制研究
结题报告
批准号:
42007104
项目类别:
青年科学基金项目
资助金额:
24.0 万元
负责人:
王建
依托单位:
学科分类:
环境土壤学
结题年份:
2023
批准年份:
2020
项目状态:
已结题
项目参与者:
王建
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中文摘要
微生物常被用于修复PAHs污染土壤。但外源微生物进入土壤后不具降解广谱性,修复效果也常不理想。PAHs污染土壤中存在着具有PAHs降解功能的土著微生物,通过直接富集驯化土著微生物获得功能菌群,进而用于原位修复PAHs污染土壤,有望克服外源微生物原位修复的技术瓶颈。遗憾地是国内外相关研究仍显薄弱,亟待开拓和深化。本项目针对制约着微生物原位修复效能的关键环节,通过富集驯化土著微生物,获得具有高效降解PAHs能力的功能土著微生物菌群;采用原位试验方法,揭示功能土著微生物菌群原位降解PAHs的基本规律;借助qPCR、16S rRNA基因高通量测序等技术,分析功能土著微生物菌群作用下土壤中PAHs代谢相关微生物和降解基因的响应,综合地阐明功能菌群原位降解土壤中PAHs的机制。试图丰富和完善人们对功能土著微生物菌群原位修复有毒有机污染土壤的理论认知,为开发经济、高效、安全的原位修复新技术提供理论支撑。
英文摘要
Bioremediation is an effective strategy in the clean-up of polycyclic aromatic hydrocarbons (PAH)-contaminated soils. However, exogenous microorganisms do not have the competitive advantage and the broad spectrum of degradation under in situ conditions, which often fail to achieve the expected restoration goals. Particular indigenous microbial flora are dominant in PAH-contaminated soil because of natural selection resulting from the pressure of PAH contaminants, and the utilization of a domesticated indigenous microbial flora as a soil inoculum may be advantageous to efficiently degrade PAHs in situ. There is a paucity of information concerning the impact of domestication indigenous microbial community on PAHs degradation in in-situ soil. To this end, this project aimed to obtain a domesticated indigenous microbial flora with a high efficiency in degrading 16 US EPA-PAHs, and their PAH degradation performances will be measured using the in situ method. The response of enzyme activities, soil microbiota and degradation genes will be elucidated using “Real-time Quantitative Gene Amplification Fluorescence Detection System” and “High Throughput Sequencing of 16S rRNA genes”, then thoroughly elucidating the processes and mechanism of the domesticated indigenous microbial flora in the colonization and in situ PAH removal in contaminated soils. The findings of this project will provide valuable information on the role of the domesticated native microbial community in the degradation of PAH in situ and will be beneficial for the development of an economically efficient, safe and new in situ remediation technology.
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DOI:10.1016/j.chemosphere.2023.140394
发表时间:2023-10
期刊:Chemosphere
影响因子:8.8
作者:Xian Zhou;Tingting Wang;Jian Wang;Shuang Chen;Wanting Ling
通讯作者:Xian Zhou;Tingting Wang;Jian Wang;Shuang Chen;Wanting Ling
DOI:10.1016/j.jhazmat.2022.129034
发表时间:2022-04
期刊:Journal of hazardous materials
影响因子:13.6
作者:Jian Wang;Z. Yang;Xian Zhou;Michael Gatheru Waigi;F. Gudda;Emmanuel Stephen Odinga;A. Mosa;W. Ling
通讯作者:Jian Wang;Z. Yang;Xian Zhou;Michael Gatheru Waigi;F. Gudda;Emmanuel Stephen Odinga;A. Mosa;W. Ling
DOI:10.1016/j.scitotenv.2020.143680
发表时间:2021
期刊:Science of the Total Environment
影响因子:9.8
作者:Wang Jian;Zhang Xiaofang;Zhou Xian;Waigi Michael Gatheru;Gudda Fredrick Owino;Zhang Chaolan;Ling Wanting
通讯作者:Ling Wanting
DOI:10.1016/j.watres.2021.116834
发表时间:2021
期刊:Water Research
影响因子:12.8
作者:Xuwen Chen;Davide Vione;Thomas Borch;Jian Wang;Yanzheng Gao
通讯作者:Yanzheng Gao
DOI:--
发表时间:2023
期刊:中国环境科学
影响因子:--
作者:林家宝;孙雨豪;王建;高彦征
通讯作者:高彦征
功能内生菌群装配对污染区蔬菜中多环芳烃衍生物的消减规律及机制
  • 批准号:
    42377011
  • 项目类别:
    面上项目
  • 资助金额:
    49万元
  • 批准年份:
    2023
  • 负责人:
    王建
  • 依托单位:
国内基金
海外基金